Vzaimodeistvie duodenzay s serpinami krovi cheloveka.

Translated title of the contribution: Interaction of duodenase with human blood serpins

N. A. Popykina, Inna Gladysheva, T. S. Zamolodchikova, N. I. Larionova

Research output: Contribution to journalArticle

Abstract

The interaction of duodenase, a new serine protease from a small group of Janus-faced proteases, with serpins, alpha 1-protease inhibitor (alpha 1-PI) and antichymotrypsin (ACT) from human blood serum, was studied. The stoichiometry of the inhibition process was found to be 1.2 and 1.3 mol/mol for alpha 1-PI and ACT, respectively. The presence of a stable enzyme-inhibitory complex duodenase-alpha 1-PI was confirmed by SDS-PAGE. No formation of the duodenase-ACT complex was demonstrated; instead, the band of the cleaved inhibitor was indicated upon the ACT hydrolysis. The suicide mechanism of the duodenase interaction with the human blood serpins was proved. The association rate constants (Ka, M-1 s-1) were 2.4 +/- 0.3 x 10(5) for alpha 1-PI and 3.0 +/- 0.4 x 10(5) for ACT. These results indicate the possibility of the regulation of duodenase activity by endogenous serpins. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2003, vol. 29, no. 6; see also http://www.maik.ru.

Original languageRussian
Pages (from-to)605-610
Number of pages6
JournalBioorganicheskaia khimiia
Volume29
Issue number6
StatePublished - Jan 1 2003

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Serpins
alpha 1-Antitrypsin
Blood
alpha 1-Antichymotrypsin
Serine Proteases
Stoichiometry
Suicide
Polyacrylamide Gel Electrophoresis
Hydrolysis
Rate constants
Peptide Hydrolases
duodenase
Association reactions
Enzymes
Serum

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Popykina, N. A., Gladysheva, I., Zamolodchikova, T. S., & Larionova, N. I. (2003). Vzaimodeistvie duodenzay s serpinami krovi cheloveka. Bioorganicheskaia khimiia, 29(6), 605-610.

Vzaimodeistvie duodenzay s serpinami krovi cheloveka. / Popykina, N. A.; Gladysheva, Inna; Zamolodchikova, T. S.; Larionova, N. I.

In: Bioorganicheskaia khimiia, Vol. 29, No. 6, 01.01.2003, p. 605-610.

Research output: Contribution to journalArticle

Popykina, NA, Gladysheva, I, Zamolodchikova, TS & Larionova, NI 2003, 'Vzaimodeistvie duodenzay s serpinami krovi cheloveka.', Bioorganicheskaia khimiia, vol. 29, no. 6, pp. 605-610.
Popykina NA, Gladysheva I, Zamolodchikova TS, Larionova NI. Vzaimodeistvie duodenzay s serpinami krovi cheloveka. Bioorganicheskaia khimiia. 2003 Jan 1;29(6):605-610.
Popykina, N. A. ; Gladysheva, Inna ; Zamolodchikova, T. S. ; Larionova, N. I. / Vzaimodeistvie duodenzay s serpinami krovi cheloveka. In: Bioorganicheskaia khimiia. 2003 ; Vol. 29, No. 6. pp. 605-610.
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