Interaction of duodenase with serpins from human blood serum

N. A. Popykina, I. P. Gladysheva, T. S. Zamolodchikova, N. I. Larionova

Research output: Contribution to journalArticle

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Abstract

The interaction of duodenase, a new serine protease from a small group of Janus-faced proteases, with serpins, α1-protease inhibitor (α1-PI) and antichymotrypsin (ACT) from human blood serum, was studied. The stoichiometry of the inhibition process was found to be 1.2 and 1.3 mol/mol for α1-PI and ACT, respectively. The presence of a stable enzyme-inhibitory complex duodenase-α1-PI was confirmed by SDS-PAGE. The formation of the duodenase-ACT complex was not demonstrated; instead, the band of the cleaved inhibitor indicated the ACT hydrolysis. The suicide mechanism of the duodenase interaction with the human blood serpins was proved. The association rate constants (ka × 105, M-1 s-1) were 2.4 ± 0.3 × 105 for α1-PI and 3.0 ± 0.4 × 105 for ACT. These results indicate the possibility of the regulation of duodenase activity by endogenous serpins.

Original languageEnglish (US)
Pages (from-to)550-555
Number of pages6
JournalRussian Journal of Bioorganic Chemistry
Volume29
Issue number6
DOIs
StatePublished - Nov 1 2003

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Serpins
Protease Inhibitors
Blood
Serum
Serine Proteases
Stoichiometry
Suicide
Polyacrylamide Gel Electrophoresis
Hydrolysis
Rate constants
Peptide Hydrolases
duodenase
Association reactions
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry

Cite this

Popykina, N. A., Gladysheva, I. P., Zamolodchikova, T. S., & Larionova, N. I. (2003). Interaction of duodenase with serpins from human blood serum. Russian Journal of Bioorganic Chemistry, 29(6), 550-555. https://doi.org/10.1023/B:RUBI.0000008895.66892.9a

Interaction of duodenase with serpins from human blood serum. / Popykina, N. A.; Gladysheva, I. P.; Zamolodchikova, T. S.; Larionova, N. I.

In: Russian Journal of Bioorganic Chemistry, Vol. 29, No. 6, 01.11.2003, p. 550-555.

Research output: Contribution to journalArticle

Popykina, NA, Gladysheva, IP, Zamolodchikova, TS & Larionova, NI 2003, 'Interaction of duodenase with serpins from human blood serum', Russian Journal of Bioorganic Chemistry, vol. 29, no. 6, pp. 550-555. https://doi.org/10.1023/B:RUBI.0000008895.66892.9a
Popykina, N. A. ; Gladysheva, I. P. ; Zamolodchikova, T. S. ; Larionova, N. I. / Interaction of duodenase with serpins from human blood serum. In: Russian Journal of Bioorganic Chemistry. 2003 ; Vol. 29, No. 6. pp. 550-555.
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