Interaction of HapX with the CCAAT-binding complex - A novel mechanism of gene regulation by iron

Peter Hortschansky, Martin Eisendle, Qusai Al Abdallah, André D. Schmidt, Sebastian Bergmann, Marcel Thön, Olaf Kniemeyer, Beate Abt, Birgit Seeber, Ernst R. Werner, Masashi Kato, Axel A. Brakhage, Hubertus Haas

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

Iron homeostasis requires subtle control systems, as iron is both essential and toxic. In Aspergillus nidulans, iron represses iron acquisition via the GATA factor SreA, and induces iron-dependent pathways at the transcriptional level, by a so far unknown mechanism. Here, we demonstrate that iron-dependent pathways (e.g., heme biosynthesis) are repressed during iron-depleted conditions by physical interaction of HapX with the CCAAT-binding core complex (CBC). Proteome analysis identified putative HapX targets. Mutual transcriptional control between hapX and sreA and synthetic lethality resulting from deletion of both regulatory genes indicate a tight interplay of these control systems. Expression of genes encoding CBC subunits was not influenced by iron availability, and their deletion was deleterious during iron-depleted and iron-replete conditions. Expression of hapX was repressed by iron and its deletion was deleterious during iron-depleted conditions only. These data indicate that the CBC has a general role and that HapX function is confined to iron-depleted conditions. Remarkably, CBC-mediated regulation has an inverse impact on the expression of the same gene set in A. nidulans, compared with Saccharomyces cerevisae.

Original languageEnglish (US)
Pages (from-to)3157-3168
Number of pages12
JournalEMBO Journal
Volume26
Issue number13
DOIs
StatePublished - Jul 11 2007

Fingerprint

Gene expression
Iron
Genes
Aspergillus nidulans
GATA Transcription Factors
Control systems
Gene Expression
Saccharomyces
Gene encoding
Aspergillus
Poisons
Biosynthesis
Proteome
Regulator Genes
Heme
Homeostasis
Availability

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Hortschansky, P., Eisendle, M., Al Abdallah, Q., Schmidt, A. D., Bergmann, S., Thön, M., ... Haas, H. (2007). Interaction of HapX with the CCAAT-binding complex - A novel mechanism of gene regulation by iron. EMBO Journal, 26(13), 3157-3168. https://doi.org/10.1038/sj.emboj.7601752

Interaction of HapX with the CCAAT-binding complex - A novel mechanism of gene regulation by iron. / Hortschansky, Peter; Eisendle, Martin; Al Abdallah, Qusai; Schmidt, André D.; Bergmann, Sebastian; Thön, Marcel; Kniemeyer, Olaf; Abt, Beate; Seeber, Birgit; Werner, Ernst R.; Kato, Masashi; Brakhage, Axel A.; Haas, Hubertus.

In: EMBO Journal, Vol. 26, No. 13, 11.07.2007, p. 3157-3168.

Research output: Contribution to journalArticle

Hortschansky, P, Eisendle, M, Al Abdallah, Q, Schmidt, AD, Bergmann, S, Thön, M, Kniemeyer, O, Abt, B, Seeber, B, Werner, ER, Kato, M, Brakhage, AA & Haas, H 2007, 'Interaction of HapX with the CCAAT-binding complex - A novel mechanism of gene regulation by iron', EMBO Journal, vol. 26, no. 13, pp. 3157-3168. https://doi.org/10.1038/sj.emboj.7601752
Hortschansky, Peter ; Eisendle, Martin ; Al Abdallah, Qusai ; Schmidt, André D. ; Bergmann, Sebastian ; Thön, Marcel ; Kniemeyer, Olaf ; Abt, Beate ; Seeber, Birgit ; Werner, Ernst R. ; Kato, Masashi ; Brakhage, Axel A. ; Haas, Hubertus. / Interaction of HapX with the CCAAT-binding complex - A novel mechanism of gene regulation by iron. In: EMBO Journal. 2007 ; Vol. 26, No. 13. pp. 3157-3168.
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