Interaction of human plasma fibronectin with cariogenic and non-cariogenic oral streptococci

Jegdish Babu, W. A. Simpson, H. S. Courtney, E. H. Beachey

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The interaction of purified human plasma fibronectin (Fn) with bacteria was studied with a variety of oral streptococci. Each of the strains of Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguis, and Streptococcus mitis tested was aggregated by Fn to various degrees, depending on the concentration of Fn added to the test mixtures. Binding assays performed with radiolabeled Fn and various strain of streptococci demonstrated various capabilities to bind Fn, and the amount of Fn bound by each strains of streptococci demonstrated various capabilities to bind Fn, and the amount of Fn bound by each strain was paralleled by its Fn-induced aggregation, with S. mutans 6715 giving the highest values in both assays. Because of the avid binding of Fn by certain strains of potentially cariogenic streptococci, we investigated the possibility that Fn may be present in human saliva and may be adsorbed from saliva into artificial tooth pellicles. Immunoreactive Fn was detected in paraffin-stimulated whose saliva by enzyme-linked immunosorbent assays of saliva-adsorbed into gelatin-coated cuvettes and by immuoelectroblots (Western blots) of salivary components separated on sodium dodecyl sulfate-polyacrylamide slab gels. Furthermore, immunoreactive Fn was found to be present in artificial tooth pellicles formed by incubating hydroxyapatite beads with whole human saliva. These results demonstrate that certain strains of oral streptococci bind to and are aggregated by Fn. The presence of Fn in artificial tooth pellicles suggests that this macromolecule may play a role in the attachment of potentially cardiogenic and other oral streptococci to dental tissues.

Original languageEnglish (US)
Pages (from-to)162-168
Number of pages7
JournalInfection and Immunity
Volume41
Issue number1
StatePublished - Jan 1 1983

Fingerprint

Streptococcus
Fibronectins
Saliva
Artificial Teeth
Streptococcus mutans
Streptococcus mitis
Streptococcus sanguis
Durapatite
Gelatin
Sodium Dodecyl Sulfate
Paraffin
Tooth

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Interaction of human plasma fibronectin with cariogenic and non-cariogenic oral streptococci. / Babu, Jegdish; Simpson, W. A.; Courtney, H. S.; Beachey, E. H.

In: Infection and Immunity, Vol. 41, No. 1, 01.01.1983, p. 162-168.

Research output: Contribution to journalArticle

Babu, J, Simpson, WA, Courtney, HS & Beachey, EH 1983, 'Interaction of human plasma fibronectin with cariogenic and non-cariogenic oral streptococci', Infection and Immunity, vol. 41, no. 1, pp. 162-168.
Babu, Jegdish ; Simpson, W. A. ; Courtney, H. S. ; Beachey, E. H. / Interaction of human plasma fibronectin with cariogenic and non-cariogenic oral streptococci. In: Infection and Immunity. 1983 ; Vol. 41, No. 1. pp. 162-168.
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