Interaction of lipoteichoic acid of group A streptococci with human platelets

E. H. Beachey, T. M. Chiang, I. Ofek, Andrew Kang

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The interaction of group A streptococcal lipoteichoic acid (LTA) with mammalian cell membranes was studied in human platelets. The binding of LTA to platelets was platelet concentration and time dependent. Binding approached a maximum within 10 min of incubation. The bound LTA could be displaced by adding a 50-fold excess of unlabeled LTA. An association constant of 1.9 x 10-7 M was calculated, and only one population of binding sites was detected. Immunoferritin labeling of LTA-treated platelets demonstrated a patchy distribution of LTA binding sites on the platelet surface. LTA inhibited collagen- and α1 chain-induced platelet aggregation, but not the platelet release reaction, suggesting that the LTA and collagen binding sites on human platelets are distinct. Apparently, LTA binds to platelets and interferes with collagen-induced aggregation although collagen is still able to attach to binding sites to trigger the release reaction.

Original languageEnglish (US)
Pages (from-to)649-654
Number of pages6
JournalInfection and Immunity
Volume16
Issue number2
StatePublished - Dec 1 1977

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Streptococcus
Blood Platelets
Collagen
Binding Sites
lipoteichoic acid
Platelet Aggregation
Cell Membrane
Population

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Interaction of lipoteichoic acid of group A streptococci with human platelets. / Beachey, E. H.; Chiang, T. M.; Ofek, I.; Kang, Andrew.

In: Infection and Immunity, Vol. 16, No. 2, 01.12.1977, p. 649-654.

Research output: Contribution to journalArticle

Beachey, EH, Chiang, TM, Ofek, I & Kang, A 1977, 'Interaction of lipoteichoic acid of group A streptococci with human platelets', Infection and Immunity, vol. 16, no. 2, pp. 649-654.
Beachey, E. H. ; Chiang, T. M. ; Ofek, I. ; Kang, Andrew. / Interaction of lipoteichoic acid of group A streptococci with human platelets. In: Infection and Immunity. 1977 ; Vol. 16, No. 2. pp. 649-654.
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