Interaction of Salivary Fibronectin with Oral Streptococci

Jegdish Babu, M. K. Dabbous

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Immunoreactive Fibronectin (Fn) has been demonstrated in stimulated human parotid saliva by western blot analysis and also found to be a component of the artificial tooth pellicles derived from hydroxyapatite (HA) beads coated with parotid saliva. Saliva depleted of gelatin-binding components showed a significantly lower degree of reactivity with anti-Fn antibodies than did the control saliva when tested by an enzyme-linked immunosorbent assay (ELISA). Depletion of gelatin-binding components from saliva was also found to affect the degree of saliva-mediated aggregation of four of the seven oral streptococci tested [Streptococcus mutans strains GS-5 and OMZ 176, S. sobrinus, and S. rattus]. Similarly, the adherence of the same four micro-organisms to the artificial tooth pellicles (derived from saliva which had previously been depleted of gelatin-binding component) was significantly inhibited ( 37-53%) when compared with the control saliva-coated HA beads. Pre-treatment of streptococci with 100 μg of soluble Fn also caused a 34—57% inhibition of adherence of the same oral streptococci to saliva-treated HA beads. Quantitation of Fn in human parotid saliva showed that the amounts of immunoreactive Fn varied from 2 to 6 μg/mL of parotid saliva. Furthermore, the Fn from parotid saliva was found to be adsorbed onto the bacterial surfaces, as demonstrated by immunofluorescence and ELISA. The presence of Fn in parotid saliva and its ability to bind to HA beads (artificial pellicles), in conjunction with the ability of soluble Fn to inhibit the adherence of streptococcal strains to the artificial tooth pellicles, suggest that the microbial ecology of the oral cavity may, in part, be influenced by the interactions mediated by salivary fibronectin.

Original languageEnglish (US)
Pages (from-to)1094-1100
Number of pages7
JournalJournal of Dental Research
Volume65
Issue number8
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Streptococcus
Saliva
Fibronectins
Artificial Teeth
Durapatite
Gelatin
Enzyme-Linked Immunosorbent Assay
Streptococcus mutans
Ecology
Fluorescent Antibody Technique
Mouth
Anti-Idiotypic Antibodies
Western Blotting

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Interaction of Salivary Fibronectin with Oral Streptococci. / Babu, Jegdish; Dabbous, M. K.

In: Journal of Dental Research, Vol. 65, No. 8, 01.01.1986, p. 1094-1100.

Research output: Contribution to journalArticle

Babu, Jegdish ; Dabbous, M. K. / Interaction of Salivary Fibronectin with Oral Streptococci. In: Journal of Dental Research. 1986 ; Vol. 65, No. 8. pp. 1094-1100.
@article{f8c10ddd4c304098b6aed2a0c7434fa1,
title = "Interaction of Salivary Fibronectin with Oral Streptococci",
abstract = "Immunoreactive Fibronectin (Fn) has been demonstrated in stimulated human parotid saliva by western blot analysis and also found to be a component of the artificial tooth pellicles derived from hydroxyapatite (HA) beads coated with parotid saliva. Saliva depleted of gelatin-binding components showed a significantly lower degree of reactivity with anti-Fn antibodies than did the control saliva when tested by an enzyme-linked immunosorbent assay (ELISA). Depletion of gelatin-binding components from saliva was also found to affect the degree of saliva-mediated aggregation of four of the seven oral streptococci tested [Streptococcus mutans strains GS-5 and OMZ 176, S. sobrinus, and S. rattus]. Similarly, the adherence of the same four micro-organisms to the artificial tooth pellicles (derived from saliva which had previously been depleted of gelatin-binding component) was significantly inhibited ( 37-53{\%}) when compared with the control saliva-coated HA beads. Pre-treatment of streptococci with 100 μg of soluble Fn also caused a 34—57{\%} inhibition of adherence of the same oral streptococci to saliva-treated HA beads. Quantitation of Fn in human parotid saliva showed that the amounts of immunoreactive Fn varied from 2 to 6 μg/mL of parotid saliva. Furthermore, the Fn from parotid saliva was found to be adsorbed onto the bacterial surfaces, as demonstrated by immunofluorescence and ELISA. The presence of Fn in parotid saliva and its ability to bind to HA beads (artificial pellicles), in conjunction with the ability of soluble Fn to inhibit the adherence of streptococcal strains to the artificial tooth pellicles, suggest that the microbial ecology of the oral cavity may, in part, be influenced by the interactions mediated by salivary fibronectin.",
author = "Jegdish Babu and Dabbous, {M. K.}",
year = "1986",
month = "1",
day = "1",
doi = "10.1177/00220345860650081001",
language = "English (US)",
volume = "65",
pages = "1094--1100",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",
number = "8",

}

TY - JOUR

T1 - Interaction of Salivary Fibronectin with Oral Streptococci

AU - Babu, Jegdish

AU - Dabbous, M. K.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - Immunoreactive Fibronectin (Fn) has been demonstrated in stimulated human parotid saliva by western blot analysis and also found to be a component of the artificial tooth pellicles derived from hydroxyapatite (HA) beads coated with parotid saliva. Saliva depleted of gelatin-binding components showed a significantly lower degree of reactivity with anti-Fn antibodies than did the control saliva when tested by an enzyme-linked immunosorbent assay (ELISA). Depletion of gelatin-binding components from saliva was also found to affect the degree of saliva-mediated aggregation of four of the seven oral streptococci tested [Streptococcus mutans strains GS-5 and OMZ 176, S. sobrinus, and S. rattus]. Similarly, the adherence of the same four micro-organisms to the artificial tooth pellicles (derived from saliva which had previously been depleted of gelatin-binding component) was significantly inhibited ( 37-53%) when compared with the control saliva-coated HA beads. Pre-treatment of streptococci with 100 μg of soluble Fn also caused a 34—57% inhibition of adherence of the same oral streptococci to saliva-treated HA beads. Quantitation of Fn in human parotid saliva showed that the amounts of immunoreactive Fn varied from 2 to 6 μg/mL of parotid saliva. Furthermore, the Fn from parotid saliva was found to be adsorbed onto the bacterial surfaces, as demonstrated by immunofluorescence and ELISA. The presence of Fn in parotid saliva and its ability to bind to HA beads (artificial pellicles), in conjunction with the ability of soluble Fn to inhibit the adherence of streptococcal strains to the artificial tooth pellicles, suggest that the microbial ecology of the oral cavity may, in part, be influenced by the interactions mediated by salivary fibronectin.

AB - Immunoreactive Fibronectin (Fn) has been demonstrated in stimulated human parotid saliva by western blot analysis and also found to be a component of the artificial tooth pellicles derived from hydroxyapatite (HA) beads coated with parotid saliva. Saliva depleted of gelatin-binding components showed a significantly lower degree of reactivity with anti-Fn antibodies than did the control saliva when tested by an enzyme-linked immunosorbent assay (ELISA). Depletion of gelatin-binding components from saliva was also found to affect the degree of saliva-mediated aggregation of four of the seven oral streptococci tested [Streptococcus mutans strains GS-5 and OMZ 176, S. sobrinus, and S. rattus]. Similarly, the adherence of the same four micro-organisms to the artificial tooth pellicles (derived from saliva which had previously been depleted of gelatin-binding component) was significantly inhibited ( 37-53%) when compared with the control saliva-coated HA beads. Pre-treatment of streptococci with 100 μg of soluble Fn also caused a 34—57% inhibition of adherence of the same oral streptococci to saliva-treated HA beads. Quantitation of Fn in human parotid saliva showed that the amounts of immunoreactive Fn varied from 2 to 6 μg/mL of parotid saliva. Furthermore, the Fn from parotid saliva was found to be adsorbed onto the bacterial surfaces, as demonstrated by immunofluorescence and ELISA. The presence of Fn in parotid saliva and its ability to bind to HA beads (artificial pellicles), in conjunction with the ability of soluble Fn to inhibit the adherence of streptococcal strains to the artificial tooth pellicles, suggest that the microbial ecology of the oral cavity may, in part, be influenced by the interactions mediated by salivary fibronectin.

UR - http://www.scopus.com/inward/record.url?scp=0022762236&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022762236&partnerID=8YFLogxK

U2 - 10.1177/00220345860650081001

DO - 10.1177/00220345860650081001

M3 - Article

VL - 65

SP - 1094

EP - 1100

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

IS - 8

ER -