Interactions of macromolecules with the mammalian cell surface

Jonathan Wall, F. Ayoub, P. O'Shea

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The characterisation of fluoresceinphosphatidylethanolamine (FPE) as a real-time indicator of the electrostatic nature of the cell membrane surface is described. The conditions appropriate for the labelling of cell membranes and the implementation of FPE as a tool to monitor the interactions of various proteins and peptides with membranes are outlined. Some complications attributed to the erythrocyte glycocalyx are examined. In addition it is shown using neuraminidase as an example, that some types of enzyme-catalysed reactions on the cell surface may be monitored in real time. It is also shown that information concerning the binding of several proteins such as serum albumin and monoclonal antibodies are accessible with this technique. The albumin in particular is shown to exhibit a saturation of binding, the analysis of which indicates that the dissociation constant for erythrocytes was determined to be 8 μM and for lymphocytes to be almost 3 μM. On the basis of this comparison together with artificial membranes, the membrane protein components of the lymphocyte surface are implicated in the binding of albumin or the erythrocyte membrane proteins reduce the affinity of the cell surface for albumin.

Original languageEnglish (US)
Pages (from-to)2673-2682
Number of pages10
JournalJournal of Cell Science
Volume108
Issue number7
StatePublished - 1995
Externally publishedYes

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Albumins
Membrane Proteins
Erythrocytes
Cell Membrane
Lymphocytes
Artificial Membranes
Glycocalyx
Erythrocyte Membrane
Neuraminidase
Static Electricity
Serum Albumin
Carrier Proteins
Monoclonal Antibodies
Peptides
Membranes
Enzymes
Proteins

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Interactions of macromolecules with the mammalian cell surface. / Wall, Jonathan; Ayoub, F.; O'Shea, P.

In: Journal of Cell Science, Vol. 108, No. 7, 1995, p. 2673-2682.

Research output: Contribution to journalArticle

Wall, J, Ayoub, F & O'Shea, P 1995, 'Interactions of macromolecules with the mammalian cell surface', Journal of Cell Science, vol. 108, no. 7, pp. 2673-2682.
Wall, Jonathan ; Ayoub, F. ; O'Shea, P. / Interactions of macromolecules with the mammalian cell surface. In: Journal of Cell Science. 1995 ; Vol. 108, No. 7. pp. 2673-2682.
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