Interclonal and intraclonal diversity among anti-DNA antibodies from an (NZB x NZW)F1 mouse

Tony Marion, D. M. Tillman, N. T. Jou

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The immunologic basis for the generation of autoantibodies that are characteristic of systemic autoimmunity in mice and humans remains obscure. Experiments directed toward the analysis of serum antibody and the cell populations that combine to generate antibody in autoimmune mice have led to the proposition that autoantibody production, including anti-DNA, results from the nonselective, polyclonal activation of B cells. The present results from the molecular analyses of anti-DNA autoantibodies from an individual (NZB x NZW)F1 autoimmune mouse, however, are inconsistent with a clonally nonselective model for autoantibody production and are most consistent with a clonally selective, Ag-driven model for anti-DNA autoantibody production. These results demonstrate that Ig V region structures contributed by germ-line V region genes; recombinational diversity, including unusual D(H) gene usage and D(H)-D(H) recombination; and somatic mutation during B cell clonal expansion are all important for generating antibody and presumably B cell Ig receptor specificity for nucleic acids including native, duplex DNA.

Original languageEnglish (US)
Pages (from-to)2322-2332
Number of pages11
JournalJournal of Immunology
Volume145
Issue number7
StatePublished - Jan 1 1990

Fingerprint

Antinuclear Antibodies
Autoantibodies
B-Lymphocytes
DNA
Antibodies
Autoimmunity
Germ Cells
Nucleic Acids
Genetic Recombination
Genes
Mutation
Serum
Population

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Interclonal and intraclonal diversity among anti-DNA antibodies from an (NZB x NZW)F1 mouse. / Marion, Tony; Tillman, D. M.; Jou, N. T.

In: Journal of Immunology, Vol. 145, No. 7, 01.01.1990, p. 2322-2332.

Research output: Contribution to journalArticle

@article{e4eeebf9a24e42999cdab75cbbe63216,
title = "Interclonal and intraclonal diversity among anti-DNA antibodies from an (NZB x NZW)F1 mouse",
abstract = "The immunologic basis for the generation of autoantibodies that are characteristic of systemic autoimmunity in mice and humans remains obscure. Experiments directed toward the analysis of serum antibody and the cell populations that combine to generate antibody in autoimmune mice have led to the proposition that autoantibody production, including anti-DNA, results from the nonselective, polyclonal activation of B cells. The present results from the molecular analyses of anti-DNA autoantibodies from an individual (NZB x NZW)F1 autoimmune mouse, however, are inconsistent with a clonally nonselective model for autoantibody production and are most consistent with a clonally selective, Ag-driven model for anti-DNA autoantibody production. These results demonstrate that Ig V region structures contributed by germ-line V region genes; recombinational diversity, including unusual D(H) gene usage and D(H)-D(H) recombination; and somatic mutation during B cell clonal expansion are all important for generating antibody and presumably B cell Ig receptor specificity for nucleic acids including native, duplex DNA.",
author = "Tony Marion and Tillman, {D. M.} and Jou, {N. T.}",
year = "1990",
month = "1",
day = "1",
language = "English (US)",
volume = "145",
pages = "2322--2332",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "7",

}

TY - JOUR

T1 - Interclonal and intraclonal diversity among anti-DNA antibodies from an (NZB x NZW)F1 mouse

AU - Marion, Tony

AU - Tillman, D. M.

AU - Jou, N. T.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - The immunologic basis for the generation of autoantibodies that are characteristic of systemic autoimmunity in mice and humans remains obscure. Experiments directed toward the analysis of serum antibody and the cell populations that combine to generate antibody in autoimmune mice have led to the proposition that autoantibody production, including anti-DNA, results from the nonselective, polyclonal activation of B cells. The present results from the molecular analyses of anti-DNA autoantibodies from an individual (NZB x NZW)F1 autoimmune mouse, however, are inconsistent with a clonally nonselective model for autoantibody production and are most consistent with a clonally selective, Ag-driven model for anti-DNA autoantibody production. These results demonstrate that Ig V region structures contributed by germ-line V region genes; recombinational diversity, including unusual D(H) gene usage and D(H)-D(H) recombination; and somatic mutation during B cell clonal expansion are all important for generating antibody and presumably B cell Ig receptor specificity for nucleic acids including native, duplex DNA.

AB - The immunologic basis for the generation of autoantibodies that are characteristic of systemic autoimmunity in mice and humans remains obscure. Experiments directed toward the analysis of serum antibody and the cell populations that combine to generate antibody in autoimmune mice have led to the proposition that autoantibody production, including anti-DNA, results from the nonselective, polyclonal activation of B cells. The present results from the molecular analyses of anti-DNA autoantibodies from an individual (NZB x NZW)F1 autoimmune mouse, however, are inconsistent with a clonally nonselective model for autoantibody production and are most consistent with a clonally selective, Ag-driven model for anti-DNA autoantibody production. These results demonstrate that Ig V region structures contributed by germ-line V region genes; recombinational diversity, including unusual D(H) gene usage and D(H)-D(H) recombination; and somatic mutation during B cell clonal expansion are all important for generating antibody and presumably B cell Ig receptor specificity for nucleic acids including native, duplex DNA.

UR - http://www.scopus.com/inward/record.url?scp=0025054661&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025054661&partnerID=8YFLogxK

M3 - Article

VL - 145

SP - 2322

EP - 2332

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 7

ER -