Interferon increases the abundance of submembranous microfilaments in HeLa-S3 cells in suspension culture

E. Wang, Lawrence Pfeffer, I. Tamm

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Human β (fibroblast) interferon inhibits the proliferation of human HeLa-S3 carcinoma cells in suspension culture. Accompanying this effect, the lateral mobility of cell surface receptors for concanavalin A is decreased and the rigidity of the plasma membrane lipid bilayer is increased. The present findings show a marked increase in the number of polymerized actin-containing microfilaments 3 days after treatment of HeLa-S3 cells with β-interferon (640 units/ml). The cortical region of the treated enlarged cells contains a thick and dense meshwork of 40-70 Å microfilaments. The actin nature of the filaments was verified by their ability to bind heavy meromyosin. These results support the concept that β-interferon induces a coordinated response in the plasma membrane and the underlying microfilaments in both tumor and normal cells.

Original languageEnglish (US)
Pages (from-to)6281-6285
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume78
Issue number10 I
DOIs
StatePublished - Jan 1 1981

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Actin Cytoskeleton
HeLa Cells
Interferons
Suspensions
Cell Membrane
Myosin Subfragments
Interferon-beta
Lipid Bilayers
Cell Surface Receptors
Membrane Lipids
Concanavalin A
Carcinoma
Neoplasms

All Science Journal Classification (ASJC) codes

  • General

Cite this

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abstract = "Human β (fibroblast) interferon inhibits the proliferation of human HeLa-S3 carcinoma cells in suspension culture. Accompanying this effect, the lateral mobility of cell surface receptors for concanavalin A is decreased and the rigidity of the plasma membrane lipid bilayer is increased. The present findings show a marked increase in the number of polymerized actin-containing microfilaments 3 days after treatment of HeLa-S3 cells with β-interferon (640 units/ml). The cortical region of the treated enlarged cells contains a thick and dense meshwork of 40-70 {\AA} microfilaments. The actin nature of the filaments was verified by their ability to bind heavy meromyosin. These results support the concept that β-interferon induces a coordinated response in the plasma membrane and the underlying microfilaments in both tumor and normal cells.",
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AU - Pfeffer, Lawrence

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