Interleukin-1β-mediated inhibition of the processes of angiogenesis in cardiac microvascular endothelial cells

Deidra Mountain, Mahipal Singh, Krishna Singh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Angiogenesis, the formation of new capillaries from preexisting vessels, plays an essential role in revascularization of the myocardium following myocardial infarction (MI). Interleukin-1β (IL-1β), a proinflammatory cytokine increased in the heart following MI, is shown to be essential for angiogenesis in the invasiveness of tumor cells, the progression of arthritic conditions and endometriosis, and the promotion of wound healing. Here we studied the steps of angiogenesis in response to IL-1β in cardiac microvascular endothelial cells (CMECs) and aortic tissue. Cell cycle progression analysis using flow cytometry indicated a G0/G1 phase cell cycle arrest in IL-1β-stimulated cells. IL-1β significantly reduced levels of fibrillar actin in the cytoskeleton, a pre-requisite for tube formation, as indicated by phalloidin-FITC staining. Wound healing assays demonstrated IL-1β prevents cell-to-cell contact formation. On the other hand, vascular endothelial growth factor-D (VEGF-D) initiated restoration of the cell monolayer. IL-1β significantly inhibited in vitro tube formation as analyzed by three-dimensional collagen matrix assay. Aortic ring assay demonstrated that IL-1β inhibits basal and VEGF-D-stimulated microvessel sprouting from aortic rings. The data presented here are novel and of significant interest, providing evidence that IL-1β impedes the process of angiogenesis in myocardial endothelial cells.

Original languageEnglish (US)
Pages (from-to)1224-1230
Number of pages7
JournalLife Sciences
Volume82
Issue number25-26
DOIs
StatePublished - Jun 20 2008

Fingerprint

Endothelial cells
Interleukin-1
Endothelial Cells
Vascular Endothelial Growth Factor D
Assays
Cells
Wound Healing
Myocardial Infarction
G1 Phase Cell Cycle Checkpoints
Phalloidine
Cell Cycle Resting Phase
Flow cytometry
Fluorescein-5-isothiocyanate
G1 Phase
Endometriosis
Microvessels
Actin Cytoskeleton
Restoration
Arthritis
Actins

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Interleukin-1β-mediated inhibition of the processes of angiogenesis in cardiac microvascular endothelial cells. / Mountain, Deidra; Singh, Mahipal; Singh, Krishna.

In: Life Sciences, Vol. 82, No. 25-26, 20.06.2008, p. 1224-1230.

Research output: Contribution to journalArticle

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