Interleukin 1β propeptide is detected intracellularly and extracellularly when human monocytes are stimulated with lps in vitro

Gloria C. Higgins, John L. Foster, Arnold Postlethwaite

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Human interleukin 1β (IL-1β) is synthesized as an inactive precursor that is cleaved by IL-1 converting enzyme (ICE) between Asp116 and Ala117 to form COOH-terminal mature IL-1β and NH2-terminal IL-1β propeptide. Little is known about the fate of the NH2-terminal cleavage product. In this study, human recombinant (hr)IL-1β propeptide (amino acids 2-116) was produced and used to prepare specific antibodies which do not recognize mature human IL-1β. These anti-propeptide antibodies were used for immunoprecipitation of biosynthetically labeled proteins from lipopolysaccharide-stimulated human monocytes. Analysis of immunopredpitates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography revealed that these antibodies recognize precursor IL-Iβ and two unique proteins: one migrating at 17.5 kD and one at 14 kD. The larger of these two proteins has a migration nearly identical to that of the recombinant IL-1β propeptide, and most likely represents naturally derived propeptide. The protein migrating at 14 kD may result from a second cleavage by ICE, between Asp27 and Gly28. These proteins accumulate intracellularly and extracellularly during pulse-chase experiments, and therefore represent stable products of precursor IL-1β cleavage.

Original languageEnglish (US)
Pages (from-to)607-614
Number of pages8
JournalJournal of Experimental Medicine
Volume180
Issue number2
DOIs
StatePublished - Aug 1 1994

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Interleukin-1
Monocytes
Proteins
Photofluorography
Caspase 1
Antibodies
In Vitro Techniques
Immunoprecipitation
Sodium Dodecyl Sulfate
Lipopolysaccharides
Polyacrylamide Gel Electrophoresis
Anti-Idiotypic Antibodies
Amino Acids
Enzymes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Interleukin 1β propeptide is detected intracellularly and extracellularly when human monocytes are stimulated with lps in vitro. / Higgins, Gloria C.; Foster, John L.; Postlethwaite, Arnold.

In: Journal of Experimental Medicine, Vol. 180, No. 2, 01.08.1994, p. 607-614.

Research output: Contribution to journalArticle

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