Interleukin 1 is an autocrine regulator of human endothelial cell growth

Federico Cozzolino, Maria Torcia, Donatella Aldinucci, Marina Ziche, Fabio Almerigogna, Daniele Bani, David Stern

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Abstract

Proliferation of endothelial cells is regulated through the autocrine production of growth factors and the expression of cognate surface receptors. In this study, we demonstrate that interleukin 1 (IL-1) is an inhibitor of endothelial growth in vitro and in vivo. IL-1 arrested growing, cultured endothelial cells in G1 phase; inhibition of proliferation was dose dependent and occurred in parallel with occupancy of endothelial surface IL-1 receptors. In an angiogenesis model, IL-1 could inhibit fibroblast growth factor-induced vessel formation. The autocrine nature of the IL-1 effect on endothelial proliferation was demonstrated by the observation that occupancy of cell-surface receptors by endogenous IL-1 depressed cell growth. The potential significance of this finding was emphasized by the detection of IL-1 in the native endothelium of human umbilical veins. A mechanism by which IL-1 may exert its inhibitory effect on endothelial cell growth was suggested by studies showing that IL-1 decreased the expression of high-affinity fibroblast growth factor binding sites on endothelium. These results point to a potentially important role of IL-1 in regulating blood vessel growth and suggest that autocrine production of inhibitory factors may be a mechanism controlling proliferation of normal cells.

Original languageEnglish (US)
Pages (from-to)6487-6491
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number17
DOIs
StatePublished - Jan 1 1990

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Interleukin-1
Endothelial Cells
Growth
Fibroblast Growth Factors
Endothelium
Growth Inhibitors
Umbilical Veins
Interleukin-1 Receptors
G1 Phase
Cell Surface Receptors
Blood Vessels
Cultured Cells
Intercellular Signaling Peptides and Proteins
Binding Sites
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • General

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Interleukin 1 is an autocrine regulator of human endothelial cell growth. / Cozzolino, Federico; Torcia, Maria; Aldinucci, Donatella; Ziche, Marina; Almerigogna, Fabio; Bani, Daniele; Stern, David.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 17, 01.01.1990, p. 6487-6491.

Research output: Contribution to journalArticle

Cozzolino, Federico ; Torcia, Maria ; Aldinucci, Donatella ; Ziche, Marina ; Almerigogna, Fabio ; Bani, Daniele ; Stern, David. / Interleukin 1 is an autocrine regulator of human endothelial cell growth. In: Proceedings of the National Academy of Sciences of the United States of America. 1990 ; Vol. 87, No. 17. pp. 6487-6491.
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AU - Bani, Daniele

AU - Stern, David

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