Interleukin-10 functions in vitro and in vivo to inhibit bacterial DNA- induced secretion of interleukin-12

Magdalena Anitescu, Jacqueline H. Chace, Rebecca Tuetken, Ae-Kyung Yi, Daniel J. Berg, Arthur M. Krieg, John S. Cowdery

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Bacterial DNA (bDNA) has a number of biologic properties, including the ability to induce interleukin-12 (IL-12) production by macrophages. We studied the role of the regulatory cytokine IL-10 as a potential inhibitor of bDNA-induced IL-12 production. IL-10 concentrations as low as 0.3 ng/ml profoundly inhibited bDNA-induced macrophage IL-12 production as measured by Elispot analysis of IL-12 p40-secreting cells. Additionally, we found that IL-10 inhibited bDNA-induced IL-12 secretion by the macrophage cell lines J774 and RAW 264. Proincubation of splenic adherent cells with IL-10 markedly reduced bDNA-induced transcription of IL12 p40 mRNA. Interestingly, after 2 h of exposure, bDNA also induces transcription of IL-10 mRNA by splenic adherent cells. The importance of IL-10 in the in vivo regulation of bDNA- induced cytokine secretion was illustrated by the response of mice with disrupted IL-10 genes (IL-10 ko mice) to i.v. bDNA challenge. Compared to +/+ mice, IL-10 knockout (ko) mice exhibited increased numbers of IL-12 and interferon-γ (IFNγ)-secreting cells following either single or repeated challenge with bDNA. These findings indicate that IL-10 plays a key role in regulating bDNA-induced production of inflammatory cytokines.

Original languageEnglish (US)
Pages (from-to)781-788
Number of pages8
JournalJournal of Interferon and Cytokine Research
Volume17
Issue number12
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

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Bacterial DNA
Interleukin-12
Interleukin-10
Macrophages
Cytokines
Knockout Mice
In Vitro Techniques
Messenger RNA
Interferons
Cell Line

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cell Biology
  • Virology

Cite this

Interleukin-10 functions in vitro and in vivo to inhibit bacterial DNA- induced secretion of interleukin-12. / Anitescu, Magdalena; Chace, Jacqueline H.; Tuetken, Rebecca; Yi, Ae-Kyung; Berg, Daniel J.; Krieg, Arthur M.; Cowdery, John S.

In: Journal of Interferon and Cytokine Research, Vol. 17, No. 12, 01.01.1997, p. 781-788.

Research output: Contribution to journalArticle

Anitescu, Magdalena ; Chace, Jacqueline H. ; Tuetken, Rebecca ; Yi, Ae-Kyung ; Berg, Daniel J. ; Krieg, Arthur M. ; Cowdery, John S. / Interleukin-10 functions in vitro and in vivo to inhibit bacterial DNA- induced secretion of interleukin-12. In: Journal of Interferon and Cytokine Research. 1997 ; Vol. 17, No. 12. pp. 781-788.
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