Interleukin-2 and interleukin-10 gene expression in calves experimentally infected with Fasciola gigantica

S. L. Ingale, P. Singh, O. K. Raina, A. K. Verma, Rudragouda Channappanavar, U. R. Mehra

Research output: Contribution to journalArticle

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Abstract

The gene expression of interleukin-2 and interleukin-10 in calves with a primary infection of Fasciola gigantica was studied. Five calves were infected orally with a dose of 1000 viable metacercariae of F. gigantica and five calves served as uninfected control animals. Expression of two cytokine genes i.e. IL-2 and IL-10 (Th1 and Th2) was measured at 10, 30 and 75 days post-infection (PI) by real-time polymerase chain reaction with the double stranded DNA-binding dye SYBR Green. Interleukin-2 was not detected in the peripheral blood mononuclear cells (PBMCs) of infected or control animals at 10, 30 and 75 days PI, however, IL-10 was present in detectable levels in PBMCs of infected animals at 10, 30 and 75 days PI, with no expression of this cytokine in the control animals. With an increased expression of IL-10 and no expression of IL-2 cytokine gene, the present study suggests that F. gigantica infection in calves evoked Th2 immune response.

Original languageEnglish (US)
Pages (from-to)141-143
Number of pages3
JournalLivestock Science
Volume131
Issue number1
DOIs
StatePublished - Jun 1 2010
Externally publishedYes

Fingerprint

Fasciola
Fasciola gigantica
interleukin-10
interleukin-2
Interleukin-10
Interleukin-2
calves
Gene Expression
gene expression
Infection
cytokines
infection
Cytokines
mononuclear leukocytes
Blood Cells
animals
dye binding
Metacercariae
metacercariae
Genes

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Interleukin-2 and interleukin-10 gene expression in calves experimentally infected with Fasciola gigantica. / Ingale, S. L.; Singh, P.; Raina, O. K.; Verma, A. K.; Channappanavar, Rudragouda; Mehra, U. R.

In: Livestock Science, Vol. 131, No. 1, 01.06.2010, p. 141-143.

Research output: Contribution to journalArticle

Ingale, S. L. ; Singh, P. ; Raina, O. K. ; Verma, A. K. ; Channappanavar, Rudragouda ; Mehra, U. R. / Interleukin-2 and interleukin-10 gene expression in calves experimentally infected with Fasciola gigantica. In: Livestock Science. 2010 ; Vol. 131, No. 1. pp. 141-143.
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