Interleukin-8 stimulates placental prostacyclin production in preeclampsia

Yuping Wang, John Baier, Charles Adair, David F. Lewis, Stephan Krueger, Tom Kruger, Michael Gurski, Edwin Brown

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

PROBLEM: Our purpose was to determine placental interleukin (IL)-8 production and its correlation with the prostacyclin production in normal and preeclamptic pregnancies and to evaluate the beneficial effect of IL-8 on prostacyclin production. METHOD OF STUDY: We determined 1) the in vitro production of IL-8 and prostacyclin by placental villous tissues from normal and preeclamptic pregnancies and 2) the production of prostacyclin by villous tissues from preeclampsia treated with recombinant human IL-8 (rhIL-8). IL-8 levels were measured by enzyme-linked immunosorbent assay and prostacyclin by radioimmmunoassay of 6-keto PGF1α, the stable metabolite of prostacyclin. RESULTS: 1) Placental production of IL-8 and 6-keto PGF1α were significantly less in preeclampsia than in normal pregnancies, P < 0.05. 2) Placental production of 6-keto PGF1α and IL-8 was significantly correlated in preeclampsia, P < 0.01. 3) Placental tissues treated with IL-8 exhibited a concentration-dependent increase in 6-keto PGF1α production. CONCLUSIONS: Placental tissues from preeclampsia produce significantly less IL-8 than tissues from normal pregnancies, which correlates with decreased prostacyclin production, IL-8 improves placental prostacyclin production in preeclampsia.

Original languageEnglish (US)
Pages (from-to)375-380
Number of pages6
JournalAmerican Journal of Reproductive Immunology
Volume42
Issue number6
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Fingerprint

Epoprostenol
Pre-Eclampsia
Interleukin-8
Pregnancy
Interleukin-6
Enzyme-Linked Immunosorbent Assay
prostaglandin F1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Interleukin-8 stimulates placental prostacyclin production in preeclampsia. / Wang, Yuping; Baier, John; Adair, Charles; Lewis, David F.; Krueger, Stephan; Kruger, Tom; Gurski, Michael; Brown, Edwin.

In: American Journal of Reproductive Immunology, Vol. 42, No. 6, 01.01.1999, p. 375-380.

Research output: Contribution to journalArticle

Wang, Yuping ; Baier, John ; Adair, Charles ; Lewis, David F. ; Krueger, Stephan ; Kruger, Tom ; Gurski, Michael ; Brown, Edwin. / Interleukin-8 stimulates placental prostacyclin production in preeclampsia. In: American Journal of Reproductive Immunology. 1999 ; Vol. 42, No. 6. pp. 375-380.
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