Interspecific differences in the expression of the AMPA-type glutamate receptors and parvalbumin in the nucleus of Edinger-Westphal of chicks and pigeons

Cláudio A.B. Toledo, Luiz R.G. Britto, Raquel S. Pires, C. L. Veenman, Anton Reiner

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The distribution of AMPA-type glutamate receptor (GluR) subunits was studied in the Edinger-Westphal nucleus (EW) of chicks and pigeons. GluR1, GluR2, GluR3 and GluR4 subunits appeared to be present in EW neurons of both species, but interspecific differences were observed in the abundance of the different types of subunits found in EW neurons. Of particular note, GluR2 immunoreactivity was present in the vast majority (ca. 80%) of neurons of pigeon EW but was found in only a small fraction (ca. 15%) of chick EW neurons. Scarcity of the GluR2 subunit in chick EW was confirmed by in situ hybridization. Because of the tendency for parvalbumin to be localized to neurons that are selectively deficient in GluR2, we also studied the localization of parvalbumin, as well as other calcium-binding proteins, in EW of chick and pigeon. Parvalbumin was found in more than 50% of chick EW neurons but was not detected in pigeon EW neurons. Our results suggest that there are major glutamatergic inputs to EW neurons in both pigeons and chicks. Furthermore, there are likely to be more AMPA-type calcium-permeable glutamate receptors in EW neurons of chick than in pigeon, since it is known that the subtype containing the edited GluR2 subunit is not calcium permeable.

Original languageEnglish (US)
Pages (from-to)122-130
Number of pages9
JournalBrain Research
Volume947
Issue number1
DOIs
StatePublished - Aug 23 2002

Fingerprint

Parvalbumins
AMPA Receptors
Columbidae
Glutamate Receptors
Neurons
Edinger-Westphal Nucleus
Calcium
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Calcium-Binding Proteins
In Situ Hybridization

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Interspecific differences in the expression of the AMPA-type glutamate receptors and parvalbumin in the nucleus of Edinger-Westphal of chicks and pigeons. / Toledo, Cláudio A.B.; Britto, Luiz R.G.; Pires, Raquel S.; Veenman, C. L.; Reiner, Anton.

In: Brain Research, Vol. 947, No. 1, 23.08.2002, p. 122-130.

Research output: Contribution to journalArticle

Toledo, Cláudio A.B. ; Britto, Luiz R.G. ; Pires, Raquel S. ; Veenman, C. L. ; Reiner, Anton. / Interspecific differences in the expression of the AMPA-type glutamate receptors and parvalbumin in the nucleus of Edinger-Westphal of chicks and pigeons. In: Brain Research. 2002 ; Vol. 947, No. 1. pp. 122-130.
@article{93863808560c495a92668f9e5e60aab1,
title = "Interspecific differences in the expression of the AMPA-type glutamate receptors and parvalbumin in the nucleus of Edinger-Westphal of chicks and pigeons",
abstract = "The distribution of AMPA-type glutamate receptor (GluR) subunits was studied in the Edinger-Westphal nucleus (EW) of chicks and pigeons. GluR1, GluR2, GluR3 and GluR4 subunits appeared to be present in EW neurons of both species, but interspecific differences were observed in the abundance of the different types of subunits found in EW neurons. Of particular note, GluR2 immunoreactivity was present in the vast majority (ca. 80{\%}) of neurons of pigeon EW but was found in only a small fraction (ca. 15{\%}) of chick EW neurons. Scarcity of the GluR2 subunit in chick EW was confirmed by in situ hybridization. Because of the tendency for parvalbumin to be localized to neurons that are selectively deficient in GluR2, we also studied the localization of parvalbumin, as well as other calcium-binding proteins, in EW of chick and pigeon. Parvalbumin was found in more than 50{\%} of chick EW neurons but was not detected in pigeon EW neurons. Our results suggest that there are major glutamatergic inputs to EW neurons in both pigeons and chicks. Furthermore, there are likely to be more AMPA-type calcium-permeable glutamate receptors in EW neurons of chick than in pigeon, since it is known that the subtype containing the edited GluR2 subunit is not calcium permeable.",
author = "Toledo, {Cl{\'a}udio A.B.} and Britto, {Luiz R.G.} and Pires, {Raquel S.} and Veenman, {C. L.} and Anton Reiner",
year = "2002",
month = "8",
day = "23",
doi = "10.1016/S0006-8993(02)02919-0",
language = "English (US)",
volume = "947",
pages = "122--130",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Interspecific differences in the expression of the AMPA-type glutamate receptors and parvalbumin in the nucleus of Edinger-Westphal of chicks and pigeons

AU - Toledo, Cláudio A.B.

AU - Britto, Luiz R.G.

AU - Pires, Raquel S.

AU - Veenman, C. L.

AU - Reiner, Anton

PY - 2002/8/23

Y1 - 2002/8/23

N2 - The distribution of AMPA-type glutamate receptor (GluR) subunits was studied in the Edinger-Westphal nucleus (EW) of chicks and pigeons. GluR1, GluR2, GluR3 and GluR4 subunits appeared to be present in EW neurons of both species, but interspecific differences were observed in the abundance of the different types of subunits found in EW neurons. Of particular note, GluR2 immunoreactivity was present in the vast majority (ca. 80%) of neurons of pigeon EW but was found in only a small fraction (ca. 15%) of chick EW neurons. Scarcity of the GluR2 subunit in chick EW was confirmed by in situ hybridization. Because of the tendency for parvalbumin to be localized to neurons that are selectively deficient in GluR2, we also studied the localization of parvalbumin, as well as other calcium-binding proteins, in EW of chick and pigeon. Parvalbumin was found in more than 50% of chick EW neurons but was not detected in pigeon EW neurons. Our results suggest that there are major glutamatergic inputs to EW neurons in both pigeons and chicks. Furthermore, there are likely to be more AMPA-type calcium-permeable glutamate receptors in EW neurons of chick than in pigeon, since it is known that the subtype containing the edited GluR2 subunit is not calcium permeable.

AB - The distribution of AMPA-type glutamate receptor (GluR) subunits was studied in the Edinger-Westphal nucleus (EW) of chicks and pigeons. GluR1, GluR2, GluR3 and GluR4 subunits appeared to be present in EW neurons of both species, but interspecific differences were observed in the abundance of the different types of subunits found in EW neurons. Of particular note, GluR2 immunoreactivity was present in the vast majority (ca. 80%) of neurons of pigeon EW but was found in only a small fraction (ca. 15%) of chick EW neurons. Scarcity of the GluR2 subunit in chick EW was confirmed by in situ hybridization. Because of the tendency for parvalbumin to be localized to neurons that are selectively deficient in GluR2, we also studied the localization of parvalbumin, as well as other calcium-binding proteins, in EW of chick and pigeon. Parvalbumin was found in more than 50% of chick EW neurons but was not detected in pigeon EW neurons. Our results suggest that there are major glutamatergic inputs to EW neurons in both pigeons and chicks. Furthermore, there are likely to be more AMPA-type calcium-permeable glutamate receptors in EW neurons of chick than in pigeon, since it is known that the subtype containing the edited GluR2 subunit is not calcium permeable.

UR - http://www.scopus.com/inward/record.url?scp=0037162979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037162979&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(02)02919-0

DO - 10.1016/S0006-8993(02)02919-0

M3 - Article

VL - 947

SP - 122

EP - 130

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -