Intervertebral disk tissue engineering using biphasic silk composite scaffolds

Sang Hyug Park, Eun Seok Gil, Hongsik Cho, Biman B. Mandal, Lee W. Tien, Byoung Hyun Min, David L. Kaplan

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Scaffolds composed of synthetic, natural, and hybrid materials have been investigated as options to restore intervertebral disk (IVD) tissue function. These systems fall short of the lamellar features of the native annulus fibrosus (AF) tissue or focus only on the nucleus pulposus (NP) tissue. However, successful regeneration of the entire IVD requires a combination approach to restore functions of both the AF and NP. To address this need, a biphasic biomaterial structure was generated by using silk protein for the AF and fibrin/hyaluronic acid (HA) gels for the NP. Two cell types, porcine AF cells and chondrocytes, were utilized. For the AF tissue, two types of scaffold morphologies, lamellar and porous, were studied with the porous system serving as a control. Toroidal scaffolds formed out of the lamellar, and porous silk materials were used to generate structures with an outer diameter of 8mm, inner diameter of 3.5mm, and a height of 3mm (the interlamellar distance in the lamellar scaffold was 150-250μm, and the average pore sizes in the porous scaffolds were 100-250μm). The scaffolds were seeded with porcine AF cells to form AF tissue, whereas porcine chondrocytes were encapsulated in fibrin/HA hydrogels for the NP tissue and embedded in the center of the toroidal disk. Histology, biochemical assays, and gene expression indicated that the lamellar scaffolds supported AF-like tissue over 2 weeks. Porcine chondrocytes formed the NP phenotype within the hydrogel after 4 weeks of culture with the AF tissue that had been previously cultured for 2 weeks, for a total of 6 weeks of cultivation. This biphasic scaffold simulating in combination of both AF and NP tissues was effective in the formation of the total IVD in vitro.

Original languageEnglish (US)
Pages (from-to)447-458
Number of pages12
JournalTissue Engineering - Part A
Volume18
Issue number5-6
DOIs
StatePublished - Mar 1 2012

Fingerprint

Silk
Intervertebral Disc
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Scaffolds
Tissue
Composite materials
Swine
Chondrocytes
Hyaluronic acid
Hyaluronic Acid
Fibrin
Hydrogels
Annulus Fibrosus
Forms (concrete)
Histology
Hydrogel
Hybrid materials
Biocompatible Materials

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Park, S. H., Gil, E. S., Cho, H., Mandal, B. B., Tien, L. W., Min, B. H., & Kaplan, D. L. (2012). Intervertebral disk tissue engineering using biphasic silk composite scaffolds. Tissue Engineering - Part A, 18(5-6), 447-458. https://doi.org/10.1089/ten.tea.2011.0195

Intervertebral disk tissue engineering using biphasic silk composite scaffolds. / Park, Sang Hyug; Gil, Eun Seok; Cho, Hongsik; Mandal, Biman B.; Tien, Lee W.; Min, Byoung Hyun; Kaplan, David L.

In: Tissue Engineering - Part A, Vol. 18, No. 5-6, 01.03.2012, p. 447-458.

Research output: Contribution to journalArticle

Park, SH, Gil, ES, Cho, H, Mandal, BB, Tien, LW, Min, BH & Kaplan, DL 2012, 'Intervertebral disk tissue engineering using biphasic silk composite scaffolds', Tissue Engineering - Part A, vol. 18, no. 5-6, pp. 447-458. https://doi.org/10.1089/ten.tea.2011.0195
Park, Sang Hyug ; Gil, Eun Seok ; Cho, Hongsik ; Mandal, Biman B. ; Tien, Lee W. ; Min, Byoung Hyun ; Kaplan, David L. / Intervertebral disk tissue engineering using biphasic silk composite scaffolds. In: Tissue Engineering - Part A. 2012 ; Vol. 18, No. 5-6. pp. 447-458.
@article{a537fd61d6e94b41b14f9813cfe4097a,
title = "Intervertebral disk tissue engineering using biphasic silk composite scaffolds",
abstract = "Scaffolds composed of synthetic, natural, and hybrid materials have been investigated as options to restore intervertebral disk (IVD) tissue function. These systems fall short of the lamellar features of the native annulus fibrosus (AF) tissue or focus only on the nucleus pulposus (NP) tissue. However, successful regeneration of the entire IVD requires a combination approach to restore functions of both the AF and NP. To address this need, a biphasic biomaterial structure was generated by using silk protein for the AF and fibrin/hyaluronic acid (HA) gels for the NP. Two cell types, porcine AF cells and chondrocytes, were utilized. For the AF tissue, two types of scaffold morphologies, lamellar and porous, were studied with the porous system serving as a control. Toroidal scaffolds formed out of the lamellar, and porous silk materials were used to generate structures with an outer diameter of 8mm, inner diameter of 3.5mm, and a height of 3mm (the interlamellar distance in the lamellar scaffold was 150-250μm, and the average pore sizes in the porous scaffolds were 100-250μm). The scaffolds were seeded with porcine AF cells to form AF tissue, whereas porcine chondrocytes were encapsulated in fibrin/HA hydrogels for the NP tissue and embedded in the center of the toroidal disk. Histology, biochemical assays, and gene expression indicated that the lamellar scaffolds supported AF-like tissue over 2 weeks. Porcine chondrocytes formed the NP phenotype within the hydrogel after 4 weeks of culture with the AF tissue that had been previously cultured for 2 weeks, for a total of 6 weeks of cultivation. This biphasic scaffold simulating in combination of both AF and NP tissues was effective in the formation of the total IVD in vitro.",
author = "Park, {Sang Hyug} and Gil, {Eun Seok} and Hongsik Cho and Mandal, {Biman B.} and Tien, {Lee W.} and Min, {Byoung Hyun} and Kaplan, {David L.}",
year = "2012",
month = "3",
day = "1",
doi = "10.1089/ten.tea.2011.0195",
language = "English (US)",
volume = "18",
pages = "447--458",
journal = "Tissue Engineering - Part A.",
issn = "1937-3341",
publisher = "Mary Ann Liebert Inc.",
number = "5-6",

}

TY - JOUR

T1 - Intervertebral disk tissue engineering using biphasic silk composite scaffolds

AU - Park, Sang Hyug

AU - Gil, Eun Seok

AU - Cho, Hongsik

AU - Mandal, Biman B.

AU - Tien, Lee W.

AU - Min, Byoung Hyun

AU - Kaplan, David L.

PY - 2012/3/1

Y1 - 2012/3/1

N2 - Scaffolds composed of synthetic, natural, and hybrid materials have been investigated as options to restore intervertebral disk (IVD) tissue function. These systems fall short of the lamellar features of the native annulus fibrosus (AF) tissue or focus only on the nucleus pulposus (NP) tissue. However, successful regeneration of the entire IVD requires a combination approach to restore functions of both the AF and NP. To address this need, a biphasic biomaterial structure was generated by using silk protein for the AF and fibrin/hyaluronic acid (HA) gels for the NP. Two cell types, porcine AF cells and chondrocytes, were utilized. For the AF tissue, two types of scaffold morphologies, lamellar and porous, were studied with the porous system serving as a control. Toroidal scaffolds formed out of the lamellar, and porous silk materials were used to generate structures with an outer diameter of 8mm, inner diameter of 3.5mm, and a height of 3mm (the interlamellar distance in the lamellar scaffold was 150-250μm, and the average pore sizes in the porous scaffolds were 100-250μm). The scaffolds were seeded with porcine AF cells to form AF tissue, whereas porcine chondrocytes were encapsulated in fibrin/HA hydrogels for the NP tissue and embedded in the center of the toroidal disk. Histology, biochemical assays, and gene expression indicated that the lamellar scaffolds supported AF-like tissue over 2 weeks. Porcine chondrocytes formed the NP phenotype within the hydrogel after 4 weeks of culture with the AF tissue that had been previously cultured for 2 weeks, for a total of 6 weeks of cultivation. This biphasic scaffold simulating in combination of both AF and NP tissues was effective in the formation of the total IVD in vitro.

AB - Scaffolds composed of synthetic, natural, and hybrid materials have been investigated as options to restore intervertebral disk (IVD) tissue function. These systems fall short of the lamellar features of the native annulus fibrosus (AF) tissue or focus only on the nucleus pulposus (NP) tissue. However, successful regeneration of the entire IVD requires a combination approach to restore functions of both the AF and NP. To address this need, a biphasic biomaterial structure was generated by using silk protein for the AF and fibrin/hyaluronic acid (HA) gels for the NP. Two cell types, porcine AF cells and chondrocytes, were utilized. For the AF tissue, two types of scaffold morphologies, lamellar and porous, were studied with the porous system serving as a control. Toroidal scaffolds formed out of the lamellar, and porous silk materials were used to generate structures with an outer diameter of 8mm, inner diameter of 3.5mm, and a height of 3mm (the interlamellar distance in the lamellar scaffold was 150-250μm, and the average pore sizes in the porous scaffolds were 100-250μm). The scaffolds were seeded with porcine AF cells to form AF tissue, whereas porcine chondrocytes were encapsulated in fibrin/HA hydrogels for the NP tissue and embedded in the center of the toroidal disk. Histology, biochemical assays, and gene expression indicated that the lamellar scaffolds supported AF-like tissue over 2 weeks. Porcine chondrocytes formed the NP phenotype within the hydrogel after 4 weeks of culture with the AF tissue that had been previously cultured for 2 weeks, for a total of 6 weeks of cultivation. This biphasic scaffold simulating in combination of both AF and NP tissues was effective in the formation of the total IVD in vitro.

UR - http://www.scopus.com/inward/record.url?scp=84857805340&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857805340&partnerID=8YFLogxK

U2 - 10.1089/ten.tea.2011.0195

DO - 10.1089/ten.tea.2011.0195

M3 - Article

VL - 18

SP - 447

EP - 458

JO - Tissue Engineering - Part A.

JF - Tissue Engineering - Part A.

SN - 1937-3341

IS - 5-6

ER -