Intestinal apolipoprotein expression in the newborn piglet

H. Wang, R. Zhan, F. Hunter, J. Du, Dennis Black

Research output: Contribution to journalArticle

Abstract

Purpose: To determine the effects of dietary fatty acids of varying chain lengths and degrees of saturation on intestinal apolipoprotein (apoLp) expression in the newborn piglet. Methods: 2-day-old female piglets received one of three isocaloric formulas containing 48% of total calories (120 kcal/kg/24 hr) as medium-chain triglycerides (TG) (MCT), intermediate-chain saturated TG (ICST), or long-chain polyunsaturated TG (LCPUT) by continuous duodenal infusion for 24 hrs. N=8 per group. After in situ radiolabeling, jejunal and ileal mucosal apo A-I and B-48 were immunoprecipitated, and synthesis expressed as % of total protein synthesis. Mucosal apo A-I and B mass was measured by ELISA as ng apoprotein/μg total protein. Jejunal apo A-IV and C-III mRNA abundance was determined by slot blot hybridization with β-actin as control. Results: 50% less apo B jejunal synthesis was present in the ICST group vs. the MCT and LCPUT groups (0.67±0.07, 1.19±0.20, and 1.25±0.15, respectively, mean±SEM, p<0.05). Jejunal apo B mass was lower only in the MCT group vs. the ICST and LCPUT groups (0.10±0.02, 0.21±0.03, and 0.16±0.03, respectively, p<0.05). Heal apo B synthesis was lowest in the ICST group (p=0.05). 2-fold higher jejunal apo A-I synthesis was found in the LCPUT group vs. the MCT and ICST groups (14.18±1.69, 7.56±2.63, and 6.36±0.58, respectively, p<0.01). No differences were found for jejunal apo A-I mass. In the ileum, the only difference was a lower apo A-I mass in the ICST group (p<0.05). Jejunal apo A-IV and C-III mRNA abundance (apoLp/β-actin ratios) was 0.81±0.09 and 1.06±0.14, respectively, in the LCPUT group, vs. 0.44±0.08 and 0.50±0.09 in the MCT group (p<0.05), and 0.42±0.06 and 0.53±0.23 in the ICST group (p<0.05). Conclusions: In the newborn piglet intestinal apoLp expression is acutely and differentially regulated by dietary lipid varying in fatty acid chain length and saturation. Patterns of regulation are complex and vary among apoLps and regions of the intestine and include pre-, co-, and post-translational mechanisms.

Original languageEnglish (US)
JournalJournal of Investigative Medicine
Volume44
Issue number1
StatePublished - Jan 1 1996
Externally publishedYes

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Apolipoproteins
Apolipoprotein A-I
Apolipoproteins B
Apolipoproteins C
Triglycerides
Chain length
Actins
Fatty Acids
Messenger RNA
Apoproteins
Ileum
Intestines
Proteins
Enzyme-Linked Immunosorbent Assay
Lipids
apolipoprotein A-IV

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Intestinal apolipoprotein expression in the newborn piglet. / Wang, H.; Zhan, R.; Hunter, F.; Du, J.; Black, Dennis.

In: Journal of Investigative Medicine, Vol. 44, No. 1, 01.01.1996.

Research output: Contribution to journalArticle

Wang, H. ; Zhan, R. ; Hunter, F. ; Du, J. ; Black, Dennis. / Intestinal apolipoprotein expression in the newborn piglet. In: Journal of Investigative Medicine. 1996 ; Vol. 44, No. 1.
@article{20f696d375844ff3989a4c523b8ba701,
title = "Intestinal apolipoprotein expression in the newborn piglet",
abstract = "Purpose: To determine the effects of dietary fatty acids of varying chain lengths and degrees of saturation on intestinal apolipoprotein (apoLp) expression in the newborn piglet. Methods: 2-day-old female piglets received one of three isocaloric formulas containing 48{\%} of total calories (120 kcal/kg/24 hr) as medium-chain triglycerides (TG) (MCT), intermediate-chain saturated TG (ICST), or long-chain polyunsaturated TG (LCPUT) by continuous duodenal infusion for 24 hrs. N=8 per group. After in situ radiolabeling, jejunal and ileal mucosal apo A-I and B-48 were immunoprecipitated, and synthesis expressed as {\%} of total protein synthesis. Mucosal apo A-I and B mass was measured by ELISA as ng apoprotein/μg total protein. Jejunal apo A-IV and C-III mRNA abundance was determined by slot blot hybridization with β-actin as control. Results: 50{\%} less apo B jejunal synthesis was present in the ICST group vs. the MCT and LCPUT groups (0.67±0.07, 1.19±0.20, and 1.25±0.15, respectively, mean±SEM, p<0.05). Jejunal apo B mass was lower only in the MCT group vs. the ICST and LCPUT groups (0.10±0.02, 0.21±0.03, and 0.16±0.03, respectively, p<0.05). Heal apo B synthesis was lowest in the ICST group (p=0.05). 2-fold higher jejunal apo A-I synthesis was found in the LCPUT group vs. the MCT and ICST groups (14.18±1.69, 7.56±2.63, and 6.36±0.58, respectively, p<0.01). No differences were found for jejunal apo A-I mass. In the ileum, the only difference was a lower apo A-I mass in the ICST group (p<0.05). Jejunal apo A-IV and C-III mRNA abundance (apoLp/β-actin ratios) was 0.81±0.09 and 1.06±0.14, respectively, in the LCPUT group, vs. 0.44±0.08 and 0.50±0.09 in the MCT group (p<0.05), and 0.42±0.06 and 0.53±0.23 in the ICST group (p<0.05). Conclusions: In the newborn piglet intestinal apoLp expression is acutely and differentially regulated by dietary lipid varying in fatty acid chain length and saturation. Patterns of regulation are complex and vary among apoLps and regions of the intestine and include pre-, co-, and post-translational mechanisms.",
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T1 - Intestinal apolipoprotein expression in the newborn piglet

AU - Wang, H.

AU - Zhan, R.

AU - Hunter, F.

AU - Du, J.

AU - Black, Dennis

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Purpose: To determine the effects of dietary fatty acids of varying chain lengths and degrees of saturation on intestinal apolipoprotein (apoLp) expression in the newborn piglet. Methods: 2-day-old female piglets received one of three isocaloric formulas containing 48% of total calories (120 kcal/kg/24 hr) as medium-chain triglycerides (TG) (MCT), intermediate-chain saturated TG (ICST), or long-chain polyunsaturated TG (LCPUT) by continuous duodenal infusion for 24 hrs. N=8 per group. After in situ radiolabeling, jejunal and ileal mucosal apo A-I and B-48 were immunoprecipitated, and synthesis expressed as % of total protein synthesis. Mucosal apo A-I and B mass was measured by ELISA as ng apoprotein/μg total protein. Jejunal apo A-IV and C-III mRNA abundance was determined by slot blot hybridization with β-actin as control. Results: 50% less apo B jejunal synthesis was present in the ICST group vs. the MCT and LCPUT groups (0.67±0.07, 1.19±0.20, and 1.25±0.15, respectively, mean±SEM, p<0.05). Jejunal apo B mass was lower only in the MCT group vs. the ICST and LCPUT groups (0.10±0.02, 0.21±0.03, and 0.16±0.03, respectively, p<0.05). Heal apo B synthesis was lowest in the ICST group (p=0.05). 2-fold higher jejunal apo A-I synthesis was found in the LCPUT group vs. the MCT and ICST groups (14.18±1.69, 7.56±2.63, and 6.36±0.58, respectively, p<0.01). No differences were found for jejunal apo A-I mass. In the ileum, the only difference was a lower apo A-I mass in the ICST group (p<0.05). Jejunal apo A-IV and C-III mRNA abundance (apoLp/β-actin ratios) was 0.81±0.09 and 1.06±0.14, respectively, in the LCPUT group, vs. 0.44±0.08 and 0.50±0.09 in the MCT group (p<0.05), and 0.42±0.06 and 0.53±0.23 in the ICST group (p<0.05). Conclusions: In the newborn piglet intestinal apoLp expression is acutely and differentially regulated by dietary lipid varying in fatty acid chain length and saturation. Patterns of regulation are complex and vary among apoLps and regions of the intestine and include pre-, co-, and post-translational mechanisms.

AB - Purpose: To determine the effects of dietary fatty acids of varying chain lengths and degrees of saturation on intestinal apolipoprotein (apoLp) expression in the newborn piglet. Methods: 2-day-old female piglets received one of three isocaloric formulas containing 48% of total calories (120 kcal/kg/24 hr) as medium-chain triglycerides (TG) (MCT), intermediate-chain saturated TG (ICST), or long-chain polyunsaturated TG (LCPUT) by continuous duodenal infusion for 24 hrs. N=8 per group. After in situ radiolabeling, jejunal and ileal mucosal apo A-I and B-48 were immunoprecipitated, and synthesis expressed as % of total protein synthesis. Mucosal apo A-I and B mass was measured by ELISA as ng apoprotein/μg total protein. Jejunal apo A-IV and C-III mRNA abundance was determined by slot blot hybridization with β-actin as control. Results: 50% less apo B jejunal synthesis was present in the ICST group vs. the MCT and LCPUT groups (0.67±0.07, 1.19±0.20, and 1.25±0.15, respectively, mean±SEM, p<0.05). Jejunal apo B mass was lower only in the MCT group vs. the ICST and LCPUT groups (0.10±0.02, 0.21±0.03, and 0.16±0.03, respectively, p<0.05). Heal apo B synthesis was lowest in the ICST group (p=0.05). 2-fold higher jejunal apo A-I synthesis was found in the LCPUT group vs. the MCT and ICST groups (14.18±1.69, 7.56±2.63, and 6.36±0.58, respectively, p<0.01). No differences were found for jejunal apo A-I mass. In the ileum, the only difference was a lower apo A-I mass in the ICST group (p<0.05). Jejunal apo A-IV and C-III mRNA abundance (apoLp/β-actin ratios) was 0.81±0.09 and 1.06±0.14, respectively, in the LCPUT group, vs. 0.44±0.08 and 0.50±0.09 in the MCT group (p<0.05), and 0.42±0.06 and 0.53±0.23 in the ICST group (p<0.05). Conclusions: In the newborn piglet intestinal apoLp expression is acutely and differentially regulated by dietary lipid varying in fatty acid chain length and saturation. Patterns of regulation are complex and vary among apoLps and regions of the intestine and include pre-, co-, and post-translational mechanisms.

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