Intestinal brush-border membrane enzyme activities and transport functions during prenatal development of pigs

Randal Buddington, Christiane Malo

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Enzyme activities and rates of leucine and glucose uptake were measured using brush-border membrane vesicles prepared from the small intestine of 7- , 8-, 10-, and 12-week fetal (43, 49, 61, and 74% of gestation) and unsuckled, neonatal pigs. Lactase was detected in 7-week fetuses, with a large increase in activity between 10 weeks of gestation and birth. γ- Glutamyltranspeptidase activity was stable throughout gestation, whereas sucrase activity was not detected. Active L-leucine uptake was already present at 7 weeks of gestation, with an increasing distal-to-proximal gradient observed at birth. D-glucose uptake was low at 7 weeks, but by 8 weeks it exhibited a typical overshoot phenomenon and established a decreasing proximal-to-distal gradient by 12 weeks. D-glucose uptake at all ages was directly related to incubation temperature, but less so for 7- and 10-week fetuses. By 12 weeks strict Na+-dependency of D-glucose uptake was observed along the entire length of the small intestine. Kinetic analysis of Na+-D-glucose cotransport showed a shift from the presence of both high and low-affinity systems at 8 weeks of gestation to a single high-affinity Michaelian component at birth. In light of similarities with human fetuses, the pig may be a valuable model for studying development of intestinal transport during gestation, particularly during the final trimester, when availability of human tissue is limited.

Original languageEnglish (US)
Pages (from-to)51-64
Number of pages14
JournalJournal of Pediatric Gastroenterology and Nutrition
Volume23
Issue number1
DOIs
StatePublished - Jul 26 1996
Externally publishedYes

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Microvilli
Swine
Pregnancy
Membranes
Glucose
Enzymes
Fetus
Parturition
Leucine
Small Intestine
Sucrase
Lactase
Temperature

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Gastroenterology

Cite this

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abstract = "Enzyme activities and rates of leucine and glucose uptake were measured using brush-border membrane vesicles prepared from the small intestine of 7- , 8-, 10-, and 12-week fetal (43, 49, 61, and 74{\%} of gestation) and unsuckled, neonatal pigs. Lactase was detected in 7-week fetuses, with a large increase in activity between 10 weeks of gestation and birth. γ- Glutamyltranspeptidase activity was stable throughout gestation, whereas sucrase activity was not detected. Active L-leucine uptake was already present at 7 weeks of gestation, with an increasing distal-to-proximal gradient observed at birth. D-glucose uptake was low at 7 weeks, but by 8 weeks it exhibited a typical overshoot phenomenon and established a decreasing proximal-to-distal gradient by 12 weeks. D-glucose uptake at all ages was directly related to incubation temperature, but less so for 7- and 10-week fetuses. By 12 weeks strict Na+-dependency of D-glucose uptake was observed along the entire length of the small intestine. Kinetic analysis of Na+-D-glucose cotransport showed a shift from the presence of both high and low-affinity systems at 8 weeks of gestation to a single high-affinity Michaelian component at birth. In light of similarities with human fetuses, the pig may be a valuable model for studying development of intestinal transport during gestation, particularly during the final trimester, when availability of human tissue is limited.",
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