Intravital fluorescence excitation in whole-animal optical imaging

Fatemeh Nooshabadi, Hee Jeong Yang, Joel N. Bixler, Ying Kong, Jeffrey D. Cirillo, Kristen C. Maitland

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Whole-animal fluorescence imaging with recombinant or fluorescently-tagged pathogens or cells enables real-time analysis of disease progression and treatment response in live animals. Tissue absorption limits penetration of fluorescence excitation light, particularly in the visible wavelength range, resulting in reduced sensitivity to deep targets. Here, we demonstrate the use of an optical fiber bundle to deliver light into the mouse lung to excite fluorescent bacteria, circumventing tissue absorption of excitation light in whole-animal imaging. We present the use of this technology to improve detection of recombinant reporter strains of td Tomato-expressing Mycobacterium bovis BCG (Bacillus Calmette Guerin) bacteria in the mouse lung. A microendoscope was integrated into a whole-animal fluorescence imager to enable intravital excitation in the mouse lung with whole-animal detection. Using this technique, the threshold of detection was measured as 103 colony forming units (CFU) during pulmonary infection. In comparison, the threshold of detection for whole-animal fluorescence imaging using standard epi-illumination was greater than 106 CFU.

Original languageEnglish (US)
Article numbere0149932
JournalPLoS ONE
Volume11
Issue number2
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

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Optical Imaging
Animals
Fluorescence
image analysis
fluorescence
Imaging techniques
lungs
Lung
animals
Mycobacterium bovis
Light
Bacteria
mice
detection limit
Stem Cells
Tissue
Mycobacterium bovis BCG
Optical Fibers
bacteria
Pathogens

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Nooshabadi, F., Yang, H. J., Bixler, J. N., Kong, Y., Cirillo, J. D., & Maitland, K. C. (2016). Intravital fluorescence excitation in whole-animal optical imaging. PLoS ONE, 11(2), [e0149932]. https://doi.org/10.1371/journal.pone.0149932

Intravital fluorescence excitation in whole-animal optical imaging. / Nooshabadi, Fatemeh; Yang, Hee Jeong; Bixler, Joel N.; Kong, Ying; Cirillo, Jeffrey D.; Maitland, Kristen C.

In: PLoS ONE, Vol. 11, No. 2, e0149932, 01.02.2016.

Research output: Contribution to journalArticle

Nooshabadi, F, Yang, HJ, Bixler, JN, Kong, Y, Cirillo, JD & Maitland, KC 2016, 'Intravital fluorescence excitation in whole-animal optical imaging', PLoS ONE, vol. 11, no. 2, e0149932. https://doi.org/10.1371/journal.pone.0149932
Nooshabadi F, Yang HJ, Bixler JN, Kong Y, Cirillo JD, Maitland KC. Intravital fluorescence excitation in whole-animal optical imaging. PLoS ONE. 2016 Feb 1;11(2). e0149932. https://doi.org/10.1371/journal.pone.0149932
Nooshabadi, Fatemeh ; Yang, Hee Jeong ; Bixler, Joel N. ; Kong, Ying ; Cirillo, Jeffrey D. ; Maitland, Kristen C. / Intravital fluorescence excitation in whole-animal optical imaging. In: PLoS ONE. 2016 ; Vol. 11, No. 2.
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