Involvement of phosphoprotein phosphatase 1 in collagen-platelet interaction

Thomas M. Chiang, Ellen S. Kang, Andrew Kang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The binding of type I collagen to its receptor initiates platelet aggregation, but the relationship of the receptor to other signal transduction components is not yet established. Correlation of platelet aggregation and anti-type I collagen receptor antibody immunoprecipitation of type I collagen treated [32PO4]-labeled platelets showed that there are two phosphoproteins (M(r) 53 kDa and 21 kDa) that coprecipitated with the 65 kDa platelet type I collagen receptor. In the present investigation, we have identified one of the phosphoproteins. A soluble component the 100,000x g supernatant fraction of 53 kDa protein is recognized by polyclonal anti-PP1 antibody. The activity of the precipitated phosphatase is inhibited by okadaic acid and inhibitor 1, suggesting that it is protein phosphatase 1 (PP 1). Phosphorylation decreases PP 1 activity as was found with [32PO4]-phosphorylase b as the substrate. The immunocoprecipitation of the type-1 collagen receptor and PP 1 inot the results of cross reactivity of the anti-type I collagen receptor antibody with the PP I protein. These results indicate that the platelet type I collagen receptor, PP 1, and unidentified 21 kDa protein are in close association with the platelet type I collagen receptor upon the binding of type I collagen by the receptor.

Original languageEnglish (US)
Pages (from-to)399-409
Number of pages11
JournalThrombosis Research
Volume84
Issue number6
DOIs
StatePublished - Dec 15 1996

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Phosphoprotein Phosphatases
Protein Phosphatase 1
Collagen
Blood Platelets
Collagen Type I
Phosphoproteins
Platelet Aggregation
Phosphorylase b
Collagen Receptors
Okadaic Acid
Proteins
Antibodies
Phosphoric Monoester Hydrolases
Immunoprecipitation
type I collagen receptor
Anti-Idiotypic Antibodies
Signal Transduction
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Involvement of phosphoprotein phosphatase 1 in collagen-platelet interaction. / Chiang, Thomas M.; Kang, Ellen S.; Kang, Andrew.

In: Thrombosis Research, Vol. 84, No. 6, 15.12.1996, p. 399-409.

Research output: Contribution to journalArticle

Chiang, Thomas M. ; Kang, Ellen S. ; Kang, Andrew. / Involvement of phosphoprotein phosphatase 1 in collagen-platelet interaction. In: Thrombosis Research. 1996 ; Vol. 84, No. 6. pp. 399-409.
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