Ion Channel Profile of TRPM8 Cold Receptors Reveals a Role of TASK-3 Potassium Channels in Thermosensation

Cruz Morenilla-Palao, Enoch Luis, Carlos Fernandez Pena Acuna, Eva Quintero, Janelle L. Weaver, Douglas A. Bayliss, Félix Viana

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Animals sense cold ambient temperatures through the activation of peripheral thermoreceptors that express TRPM8, a cold- and menthol-activated ion channel. These receptors can discriminate a very wide range of temperatures from innocuous to noxious. The molecular mechanism responsible for the variable sensitivity of individual cold receptors to temperature is unclear. To address this question, we performed a detailed ion channel expression analysis of cold-sensitive neurons, combining bacterial artificial chromosome (BAC) transgenesis with a molecular-profiling approach in fluorescence-activated cell sorting (FACS)-purified TRPM8 neurons. We found that TASK-3 leak potassium channels are highly enriched in a subpopulation of these sensory neurons. The thermal threshold of TRPM8 cold neurons is decreased during TASK-3 blockade and in mice lacking TASK-3, and, most importantly, these mice display hypersensitivity to cold. Our results demonstrate a role of TASK-3 channels in thermosensation, showing that a channel-based combinatorial strategy in TRPM8 cold thermoreceptors leads to molecular specialization and functional diversity.

Original languageEnglish (US)
Pages (from-to)1571-1582
Number of pages12
JournalCell Reports
Volume8
Issue number5
DOIs
StatePublished - Jan 1 2014

Fingerprint

Potassium Channels
Ion Channels
Neurons
Thermoreceptors
Tandem Pore Domain Potassium Channels
Menthol
Chromosomes
Sorting
Temperature
Bacterial Artificial Chromosomes
Gene Transfer Techniques
Animals
Fluorescence
Chemical activation
Cells
Sensory Receptor Cells
Flow Cytometry
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ion Channel Profile of TRPM8 Cold Receptors Reveals a Role of TASK-3 Potassium Channels in Thermosensation. / Morenilla-Palao, Cruz; Luis, Enoch; Fernandez Pena Acuna, Carlos; Quintero, Eva; Weaver, Janelle L.; Bayliss, Douglas A.; Viana, Félix.

In: Cell Reports, Vol. 8, No. 5, 01.01.2014, p. 1571-1582.

Research output: Contribution to journalArticle

Morenilla-Palao, Cruz ; Luis, Enoch ; Fernandez Pena Acuna, Carlos ; Quintero, Eva ; Weaver, Janelle L. ; Bayliss, Douglas A. ; Viana, Félix. / Ion Channel Profile of TRPM8 Cold Receptors Reveals a Role of TASK-3 Potassium Channels in Thermosensation. In: Cell Reports. 2014 ; Vol. 8, No. 5. pp. 1571-1582.
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