Ion transport properties of fetal sheep alveolar epithelial cells in monolayer culture

Gussie J. Tessier, Guy D. Lester, Max Langham, Sidney Cassin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Alveolar type II cells were isolated from late-term fetal sheep to investigate ion transport across fetal distal lung epithelium. In Ussing chambers, basal transepithelial potential difference (PD; reference apical side), equivalent short-circuit current (I(eq)), and resistance were -0.10 ± 0.05 mV, 0.10 ± 0.08 μA/cm2, and 821.5 ± 38.8Ω · cm2, respectively. Epinephrine (100 nM) increased PD from -0.13 ± 0.19 to -1.37 ± 0.20 mV and I(eq) from 0.18 ± 0.26 to 1.47 ± 0.28 μA/cm2. Propranolol (100 nM) inhibited responses to epinephrine. Forskolin (10 μM) increased PD to -0.81 ± 0.08 mV and I(eq) to 1.02 ± 0.12 μA/cm2. Mucosal amiloride (200 μM) and serosal bumetanide (10 μM) decreased the forskolin-stimulated PD by 23.42 ± 4.73 and 25.57 ± 3.9%, respectively. We conclude that in fetal sheep distal lung epithelium amiloride-inhibitable sodium absorption and bumetanide-sensitive chloride secretion are stimulated by forskolin and that epinephrine effects on ion transport are mediated by β-adrenergic receptors.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume270
Issue number6 14-6
StatePublished - Jun 1 1996

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Alveolar Epithelial Cells
Ion Transport
Colforsin
Epinephrine
Bumetanide
Sheep
Amiloride
Epithelium
Lung
Propranolol
Adrenergic Receptors
Chlorides
Sodium

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Ion transport properties of fetal sheep alveolar epithelial cells in monolayer culture. / Tessier, Gussie J.; Lester, Guy D.; Langham, Max; Cassin, Sidney.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 270, No. 6 14-6, 01.06.1996.

Research output: Contribution to journalArticle

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