Isoflurane-induced changes in righting response and breathing are modulated by RGS proteins

Eduardo E. Icaza, Xinyan Huang, Ying Fu, Richard R. Neubig, Helen Baghdoyan, Ralph Lydic

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

BACKGROUND:: Recent evidence suggests that G protein-coupled receptors, especially those linked to Gαi, contribute to the mechanisms of anesthetic action. Regulator of G protein signaling (RGS) proteins bind to activated Gαi and inhibit signal transduction. Genomic knock-in mice with an RGS-insensitive Gαi2 G184S (Gαi2 GS) allele exhibit enhanced Gαi2 signaling and provide a novel approach for investigating the role of Gαi2 signaling and RGS proteins in general anesthesia. METHODS:: We anesthetized homozygous Gαi2 GS/GS and wild-type (WT) mice with isoflurane and quantified time (in seconds) to loss and resumption of righting response. During recovery from isoflurane anesthesia, breathing was quantified in a plethysmography chamber for both lines of mice. RESULTS:: Gαi2 GS/GS mice required significantly less time for loss of righting and significantly more time for resumption of righting than WT mice. During recovery from isoflurane anesthesia, Gαi2 GS/GS mice exhibited significantly greater respiratory depression. Poincaré analyses show that GS/GS mice have diminished respiratory variability compared with WT mice. CONCLUSION:: Modulation of Gαi2 signaling by RGS proteins alters loss and resumption of wakefulness and state-dependent changes in breathing.

Original languageEnglish (US)
Pages (from-to)1500-1505
Number of pages6
JournalAnesthesia and analgesia
Volume109
Issue number5
DOIs
StatePublished - Jan 1 2009

Fingerprint

RGS Proteins
Righting Reflex
Isoflurane
Respiration
Anesthesia
GTP-Binding Protein Regulators
Plethysmography
Wakefulness
G-Protein-Coupled Receptors
Respiratory Insufficiency
General Anesthesia
Anesthetics
Signal Transduction
Alleles

All Science Journal Classification (ASJC) codes

  • Anesthesiology and Pain Medicine

Cite this

Isoflurane-induced changes in righting response and breathing are modulated by RGS proteins. / Icaza, Eduardo E.; Huang, Xinyan; Fu, Ying; Neubig, Richard R.; Baghdoyan, Helen; Lydic, Ralph.

In: Anesthesia and analgesia, Vol. 109, No. 5, 01.01.2009, p. 1500-1505.

Research output: Contribution to journalArticle

Icaza, Eduardo E. ; Huang, Xinyan ; Fu, Ying ; Neubig, Richard R. ; Baghdoyan, Helen ; Lydic, Ralph. / Isoflurane-induced changes in righting response and breathing are modulated by RGS proteins. In: Anesthesia and analgesia. 2009 ; Vol. 109, No. 5. pp. 1500-1505.
@article{c52e92999c764249b39a4cf6c37b92eb,
title = "Isoflurane-induced changes in righting response and breathing are modulated by RGS proteins",
abstract = "BACKGROUND:: Recent evidence suggests that G protein-coupled receptors, especially those linked to Gαi, contribute to the mechanisms of anesthetic action. Regulator of G protein signaling (RGS) proteins bind to activated Gαi and inhibit signal transduction. Genomic knock-in mice with an RGS-insensitive Gαi2 G184S (Gαi2 GS) allele exhibit enhanced Gαi2 signaling and provide a novel approach for investigating the role of Gαi2 signaling and RGS proteins in general anesthesia. METHODS:: We anesthetized homozygous Gαi2 GS/GS and wild-type (WT) mice with isoflurane and quantified time (in seconds) to loss and resumption of righting response. During recovery from isoflurane anesthesia, breathing was quantified in a plethysmography chamber for both lines of mice. RESULTS:: Gαi2 GS/GS mice required significantly less time for loss of righting and significantly more time for resumption of righting than WT mice. During recovery from isoflurane anesthesia, Gαi2 GS/GS mice exhibited significantly greater respiratory depression. Poincar{\'e} analyses show that GS/GS mice have diminished respiratory variability compared with WT mice. CONCLUSION:: Modulation of Gαi2 signaling by RGS proteins alters loss and resumption of wakefulness and state-dependent changes in breathing.",
author = "Icaza, {Eduardo E.} and Xinyan Huang and Ying Fu and Neubig, {Richard R.} and Helen Baghdoyan and Ralph Lydic",
year = "2009",
month = "1",
day = "1",
doi = "10.1213/ANE.0b013e3181ba7815",
language = "English (US)",
volume = "109",
pages = "1500--1505",
journal = "Anesthesia and Analgesia",
issn = "0003-2999",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Isoflurane-induced changes in righting response and breathing are modulated by RGS proteins

AU - Icaza, Eduardo E.

AU - Huang, Xinyan

AU - Fu, Ying

AU - Neubig, Richard R.

AU - Baghdoyan, Helen

AU - Lydic, Ralph

PY - 2009/1/1

Y1 - 2009/1/1

N2 - BACKGROUND:: Recent evidence suggests that G protein-coupled receptors, especially those linked to Gαi, contribute to the mechanisms of anesthetic action. Regulator of G protein signaling (RGS) proteins bind to activated Gαi and inhibit signal transduction. Genomic knock-in mice with an RGS-insensitive Gαi2 G184S (Gαi2 GS) allele exhibit enhanced Gαi2 signaling and provide a novel approach for investigating the role of Gαi2 signaling and RGS proteins in general anesthesia. METHODS:: We anesthetized homozygous Gαi2 GS/GS and wild-type (WT) mice with isoflurane and quantified time (in seconds) to loss and resumption of righting response. During recovery from isoflurane anesthesia, breathing was quantified in a plethysmography chamber for both lines of mice. RESULTS:: Gαi2 GS/GS mice required significantly less time for loss of righting and significantly more time for resumption of righting than WT mice. During recovery from isoflurane anesthesia, Gαi2 GS/GS mice exhibited significantly greater respiratory depression. Poincaré analyses show that GS/GS mice have diminished respiratory variability compared with WT mice. CONCLUSION:: Modulation of Gαi2 signaling by RGS proteins alters loss and resumption of wakefulness and state-dependent changes in breathing.

AB - BACKGROUND:: Recent evidence suggests that G protein-coupled receptors, especially those linked to Gαi, contribute to the mechanisms of anesthetic action. Regulator of G protein signaling (RGS) proteins bind to activated Gαi and inhibit signal transduction. Genomic knock-in mice with an RGS-insensitive Gαi2 G184S (Gαi2 GS) allele exhibit enhanced Gαi2 signaling and provide a novel approach for investigating the role of Gαi2 signaling and RGS proteins in general anesthesia. METHODS:: We anesthetized homozygous Gαi2 GS/GS and wild-type (WT) mice with isoflurane and quantified time (in seconds) to loss and resumption of righting response. During recovery from isoflurane anesthesia, breathing was quantified in a plethysmography chamber for both lines of mice. RESULTS:: Gαi2 GS/GS mice required significantly less time for loss of righting and significantly more time for resumption of righting than WT mice. During recovery from isoflurane anesthesia, Gαi2 GS/GS mice exhibited significantly greater respiratory depression. Poincaré analyses show that GS/GS mice have diminished respiratory variability compared with WT mice. CONCLUSION:: Modulation of Gαi2 signaling by RGS proteins alters loss and resumption of wakefulness and state-dependent changes in breathing.

UR - http://www.scopus.com/inward/record.url?scp=70350736250&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350736250&partnerID=8YFLogxK

U2 - 10.1213/ANE.0b013e3181ba7815

DO - 10.1213/ANE.0b013e3181ba7815

M3 - Article

VL - 109

SP - 1500

EP - 1505

JO - Anesthesia and Analgesia

JF - Anesthesia and Analgesia

SN - 0003-2999

IS - 5

ER -