Isoforms of secretory group two phospholipase a (sPLA2) in mouse ocular surface epithelia and lacrimal glands

Yi Wei, Alexander Pinhas, Ying Liu, Seth Epstein, Ju Wang, Penny Asbell

Research output: Contribution to journalArticle

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Abstract

PURPOSE. To compare and contrast the distribution patterns of select secretory group two phospholipase A (sPLA2) isoforms in corneal epithelia (CN), conjunctival epithelia (CNJ), and lacrimal glands (LG) of BALB/c and C57BL/6 mice. METHODS. Gene expression of select sPLA2 isoforms was quantified via real-time reverse-transcription PCR (qRT 2-PCR). Immunofluorescence assay (IFA) of the sPLA2-IIa, -V, and -X isoforms were used to confirm qRT 2-PCR results. sPLA2-IIa function was confirmed via in vitro CN and CNJ culturing. RESULTS. qRT 2-PCR revealed that sPLA2 isoforms (pla2g5, 12a, and 12b), cPLA2 isoform (pla2g4a), iPLA2 isoform (pla2g6), and PLA2-receptor (pla2r1) were present in all tissues of both strains, whereas sPLA2 isoforms (pla2g1b, 2e, and 3) were absent. sPLA2 isoforms (pla2g2a, 2d, 2f, and 10) showed tissue- and strain-specific expression: 2a in BALB/c CNJ only; 2d at higher levels in CNJ than LG; and 2f and 10 in CN and CNJ, but absent in LG. Upon dry eye (DE) induction, pla2g2a, 2d, and 2f were upregulated in BALB/c CNJ, and 10 was absent from CN. Furthermore, BALB/c DE mice showed upregulation of pla2r1 in CN and CNJ and downregulation of 12a and 12b in LG. IFA of sPLA2-IIa, -V, and -X in DE CNJ confirmed the upregulation of pla2g2a, 5, and 10. Last, in vitro CN and CNJ culturing confirmed that sPLA2-IIa amplifies ocular surface inflammation in CNJ but not in CN. CONCLUSIONS. sPLA2 isoforms exhibit differential expression patterns when comparing BALB/c with C57BL/6 mice; and DE with control BALB/c mice. These findings suggest that at least some sPLA2 isoforms must have significant roles in ocular surface physiology and inflammation.

Original languageEnglish (US)
Pages (from-to)2845-2855
Number of pages11
JournalInvestigative Ophthalmology and Visual Science
Volume53
Issue number6
DOIs
StatePublished - May 1 2012

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Lacrimal Apparatus
Phospholipases
Corneal Epithelium
Protein Isoforms
Epithelium
Polymerase Chain Reaction
Inbred C57BL Mouse
Fluorescent Antibody Technique
Up-Regulation
Ocular Physiological Phenomena
Inflammation
Phospholipases A
Reverse Transcription
Down-Regulation
Gene Expression

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Isoforms of secretory group two phospholipase a (sPLA2) in mouse ocular surface epithelia and lacrimal glands. / Wei, Yi; Pinhas, Alexander; Liu, Ying; Epstein, Seth; Wang, Ju; Asbell, Penny.

In: Investigative Ophthalmology and Visual Science, Vol. 53, No. 6, 01.05.2012, p. 2845-2855.

Research output: Contribution to journalArticle

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title = "Isoforms of secretory group two phospholipase a (sPLA2) in mouse ocular surface epithelia and lacrimal glands",
abstract = "PURPOSE. To compare and contrast the distribution patterns of select secretory group two phospholipase A (sPLA2) isoforms in corneal epithelia (CN), conjunctival epithelia (CNJ), and lacrimal glands (LG) of BALB/c and C57BL/6 mice. METHODS. Gene expression of select sPLA2 isoforms was quantified via real-time reverse-transcription PCR (qRT 2-PCR). Immunofluorescence assay (IFA) of the sPLA2-IIa, -V, and -X isoforms were used to confirm qRT 2-PCR results. sPLA2-IIa function was confirmed via in vitro CN and CNJ culturing. RESULTS. qRT 2-PCR revealed that sPLA2 isoforms (pla2g5, 12a, and 12b), cPLA2 isoform (pla2g4a), iPLA2 isoform (pla2g6), and PLA2-receptor (pla2r1) were present in all tissues of both strains, whereas sPLA2 isoforms (pla2g1b, 2e, and 3) were absent. sPLA2 isoforms (pla2g2a, 2d, 2f, and 10) showed tissue- and strain-specific expression: 2a in BALB/c CNJ only; 2d at higher levels in CNJ than LG; and 2f and 10 in CN and CNJ, but absent in LG. Upon dry eye (DE) induction, pla2g2a, 2d, and 2f were upregulated in BALB/c CNJ, and 10 was absent from CN. Furthermore, BALB/c DE mice showed upregulation of pla2r1 in CN and CNJ and downregulation of 12a and 12b in LG. IFA of sPLA2-IIa, -V, and -X in DE CNJ confirmed the upregulation of pla2g2a, 5, and 10. Last, in vitro CN and CNJ culturing confirmed that sPLA2-IIa amplifies ocular surface inflammation in CNJ but not in CN. CONCLUSIONS. sPLA2 isoforms exhibit differential expression patterns when comparing BALB/c with C57BL/6 mice; and DE with control BALB/c mice. These findings suggest that at least some sPLA2 isoforms must have significant roles in ocular surface physiology and inflammation.",
author = "Yi Wei and Alexander Pinhas and Ying Liu and Seth Epstein and Ju Wang and Penny Asbell",
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T1 - Isoforms of secretory group two phospholipase a (sPLA2) in mouse ocular surface epithelia and lacrimal glands

AU - Wei, Yi

AU - Pinhas, Alexander

AU - Liu, Ying

AU - Epstein, Seth

AU - Wang, Ju

AU - Asbell, Penny

PY - 2012/5/1

Y1 - 2012/5/1

N2 - PURPOSE. To compare and contrast the distribution patterns of select secretory group two phospholipase A (sPLA2) isoforms in corneal epithelia (CN), conjunctival epithelia (CNJ), and lacrimal glands (LG) of BALB/c and C57BL/6 mice. METHODS. Gene expression of select sPLA2 isoforms was quantified via real-time reverse-transcription PCR (qRT 2-PCR). Immunofluorescence assay (IFA) of the sPLA2-IIa, -V, and -X isoforms were used to confirm qRT 2-PCR results. sPLA2-IIa function was confirmed via in vitro CN and CNJ culturing. RESULTS. qRT 2-PCR revealed that sPLA2 isoforms (pla2g5, 12a, and 12b), cPLA2 isoform (pla2g4a), iPLA2 isoform (pla2g6), and PLA2-receptor (pla2r1) were present in all tissues of both strains, whereas sPLA2 isoforms (pla2g1b, 2e, and 3) were absent. sPLA2 isoforms (pla2g2a, 2d, 2f, and 10) showed tissue- and strain-specific expression: 2a in BALB/c CNJ only; 2d at higher levels in CNJ than LG; and 2f and 10 in CN and CNJ, but absent in LG. Upon dry eye (DE) induction, pla2g2a, 2d, and 2f were upregulated in BALB/c CNJ, and 10 was absent from CN. Furthermore, BALB/c DE mice showed upregulation of pla2r1 in CN and CNJ and downregulation of 12a and 12b in LG. IFA of sPLA2-IIa, -V, and -X in DE CNJ confirmed the upregulation of pla2g2a, 5, and 10. Last, in vitro CN and CNJ culturing confirmed that sPLA2-IIa amplifies ocular surface inflammation in CNJ but not in CN. CONCLUSIONS. sPLA2 isoforms exhibit differential expression patterns when comparing BALB/c with C57BL/6 mice; and DE with control BALB/c mice. These findings suggest that at least some sPLA2 isoforms must have significant roles in ocular surface physiology and inflammation.

AB - PURPOSE. To compare and contrast the distribution patterns of select secretory group two phospholipase A (sPLA2) isoforms in corneal epithelia (CN), conjunctival epithelia (CNJ), and lacrimal glands (LG) of BALB/c and C57BL/6 mice. METHODS. Gene expression of select sPLA2 isoforms was quantified via real-time reverse-transcription PCR (qRT 2-PCR). Immunofluorescence assay (IFA) of the sPLA2-IIa, -V, and -X isoforms were used to confirm qRT 2-PCR results. sPLA2-IIa function was confirmed via in vitro CN and CNJ culturing. RESULTS. qRT 2-PCR revealed that sPLA2 isoforms (pla2g5, 12a, and 12b), cPLA2 isoform (pla2g4a), iPLA2 isoform (pla2g6), and PLA2-receptor (pla2r1) were present in all tissues of both strains, whereas sPLA2 isoforms (pla2g1b, 2e, and 3) were absent. sPLA2 isoforms (pla2g2a, 2d, 2f, and 10) showed tissue- and strain-specific expression: 2a in BALB/c CNJ only; 2d at higher levels in CNJ than LG; and 2f and 10 in CN and CNJ, but absent in LG. Upon dry eye (DE) induction, pla2g2a, 2d, and 2f were upregulated in BALB/c CNJ, and 10 was absent from CN. Furthermore, BALB/c DE mice showed upregulation of pla2r1 in CN and CNJ and downregulation of 12a and 12b in LG. IFA of sPLA2-IIa, -V, and -X in DE CNJ confirmed the upregulation of pla2g2a, 5, and 10. Last, in vitro CN and CNJ culturing confirmed that sPLA2-IIa amplifies ocular surface inflammation in CNJ but not in CN. CONCLUSIONS. sPLA2 isoforms exhibit differential expression patterns when comparing BALB/c with C57BL/6 mice; and DE with control BALB/c mice. These findings suggest that at least some sPLA2 isoforms must have significant roles in ocular surface physiology and inflammation.

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