Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice

Takayuki Nakagomi, Akihiko Taguchi, Yoshihiro Fujimori, Orie Saino, Akiko Nakano-Doi, Shuji Kubo, Akinobu Gotoh, Toshihiro Soma, Hiroo Yoshikawa, Tomoyuki Nishizaki, Nami Nakagomi, David Stern, Tomohiro Matsuyama

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The CNS has the potential to marshal strong reparative mechanisms, including activation of endogenous neurogenesis, after a brain injury such as stroke. However, the response of neural stem/progenitor cells to stroke is poorly understood. Recently, neural stem/progenitor cells have been identified in the cerebral cortex, as well as previously recognized regions such as the subventricular or subgranular zones of the hippocampus, suggesting that a contribution of cortex-derived neural stem/progenitor cells may repair ischemic lesions of the cerebral cortex. In the present study, using a highly reproducible murine model of cortical infarction, we have found nestin-positive cells in the post-stroke cerebral cortex, but not in the non-ischemic cortex. Cells obtained from the ischemic core of the post-stroke cerebral cortex formed neurosphere-like cell clusters expressing nestin; such cells had the capacity for self-renewal and differentiated into electrophysiologically functional neurons, astrocytes and myelin-producing oligodendrocytes. Nestin-positive cells from the stroke-affected cortex migrated into the peri-infarct area and differentiated into glial cells in vivo. Although we could not detect differentiation of nestin-positive cells into neurons in vivo, our current observations indicate that endogenous neural stem/progenitors with the potential to become neurons can develop within post-stroke cerebral cortex.

Original languageEnglish (US)
Pages (from-to)1842-1852
Number of pages11
JournalEuropean Journal of Neuroscience
Volume29
Issue number9
DOIs
StatePublished - May 2009
Externally publishedYes

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Neural Stem Cells
Cerebral Cortex
Nestin
Stem Cells
Stroke
Neurons
Neurogenesis
Oligodendroglia
Myelin Sheath
Neuroglia
Astrocytes
Brain Injuries
Infarction
Hippocampus

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Nakagomi, T., Taguchi, A., Fujimori, Y., Saino, O., Nakano-Doi, A., Kubo, S., ... Matsuyama, T. (2009). Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice. European Journal of Neuroscience, 29(9), 1842-1852. https://doi.org/10.1111/j.1460-9568.2009.06732.x

Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice. / Nakagomi, Takayuki; Taguchi, Akihiko; Fujimori, Yoshihiro; Saino, Orie; Nakano-Doi, Akiko; Kubo, Shuji; Gotoh, Akinobu; Soma, Toshihiro; Yoshikawa, Hiroo; Nishizaki, Tomoyuki; Nakagomi, Nami; Stern, David; Matsuyama, Tomohiro.

In: European Journal of Neuroscience, Vol. 29, No. 9, 05.2009, p. 1842-1852.

Research output: Contribution to journalArticle

Nakagomi, T, Taguchi, A, Fujimori, Y, Saino, O, Nakano-Doi, A, Kubo, S, Gotoh, A, Soma, T, Yoshikawa, H, Nishizaki, T, Nakagomi, N, Stern, D & Matsuyama, T 2009, 'Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice', European Journal of Neuroscience, vol. 29, no. 9, pp. 1842-1852. https://doi.org/10.1111/j.1460-9568.2009.06732.x
Nakagomi, Takayuki ; Taguchi, Akihiko ; Fujimori, Yoshihiro ; Saino, Orie ; Nakano-Doi, Akiko ; Kubo, Shuji ; Gotoh, Akinobu ; Soma, Toshihiro ; Yoshikawa, Hiroo ; Nishizaki, Tomoyuki ; Nakagomi, Nami ; Stern, David ; Matsuyama, Tomohiro. / Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice. In: European Journal of Neuroscience. 2009 ; Vol. 29, No. 9. pp. 1842-1852.
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