Isolation and Characterization of Soybean Bowman-Birk Inhibitor from Different Sources

I. P. Gladysheva, N. G. Balabushevich, N. A. Moroz, N. I. Larionova

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A chromatographic procedure for isolation of different isoforms of Bowman-Birk soybean trypsin inhibitors was developed. The number of isoforms was shown to depend on soybean cultivar. The amount of the classical Bowman-Birk inhibitor (BBI) in different soybean cultivars, commercial flour, and processing products was analyzed. BBI reaches its highest concentration in freshly milled seeds. Storage conditions optimum for preservation of maximum inhibitory activity in soybean raw material were developed. The use of indirect enzyme immunoassay for BBI detection during its isolation from different sources was demonstrated.

Original languageEnglish (US)
Pages (from-to)198-203
Number of pages6
JournalBiochemistry (Moscow)
Volume65
Issue number2
StatePublished - Feb 1 2000

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Soybeans
Bowman Birk Soybean Trypsin Inhibitor
Protein Isoforms
Seed
Raw materials
Flour
Immunoenzyme Techniques
Seeds
Enzymes
Processing

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Gladysheva, I. P., Balabushevich, N. G., Moroz, N. A., & Larionova, N. I. (2000). Isolation and Characterization of Soybean Bowman-Birk Inhibitor from Different Sources. Biochemistry (Moscow), 65(2), 198-203.

Isolation and Characterization of Soybean Bowman-Birk Inhibitor from Different Sources. / Gladysheva, I. P.; Balabushevich, N. G.; Moroz, N. A.; Larionova, N. I.

In: Biochemistry (Moscow), Vol. 65, No. 2, 01.02.2000, p. 198-203.

Research output: Contribution to journalArticle

Gladysheva, IP, Balabushevich, NG, Moroz, NA & Larionova, NI 2000, 'Isolation and Characterization of Soybean Bowman-Birk Inhibitor from Different Sources', Biochemistry (Moscow), vol. 65, no. 2, pp. 198-203.
Gladysheva IP, Balabushevich NG, Moroz NA, Larionova NI. Isolation and Characterization of Soybean Bowman-Birk Inhibitor from Different Sources. Biochemistry (Moscow). 2000 Feb 1;65(2):198-203.
Gladysheva, I. P. ; Balabushevich, N. G. ; Moroz, N. A. ; Larionova, N. I. / Isolation and Characterization of Soybean Bowman-Birk Inhibitor from Different Sources. In: Biochemistry (Moscow). 2000 ; Vol. 65, No. 2. pp. 198-203.
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