Isolation, characterization, and differentiation of dental pulp stem cells in ferrets

Negar Homayounfar, Prashant Verma, Ali Nosrat, Ikbale El Ayachi, Zongdong Yu, Elaine Romberg, George Huang, Ashraf F. Fouad

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Abstract

Introduction The ferret canine tooth has been introduced as a suitable model for studying dental pulp regeneration. The aim of this study was to isolate and characterize ferret dental pulp stem cells (fDPSCs) and their differentiation potential. Methods Dental pulp stem cells were isolated from freshly extracted ferret canine teeth. The cells were examined for the expression of stem cell markers STRO-1, CD90, CD105, and CD146. The osteo/odontogenic and adipogenic differentiation potential of fDPSCs was evaluated. Osteogenic and odontogenic marker genes were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) on days 1, 4, and 8 after osteo/odontogenic induction of fDPSCs including dentin sialophosphoprotein (DSPP), dentin matrix protein-1, osteopontin, and alkaline phosphatase. Human dental pulp cells were used as the control. The results were analyzed using 3-way analysis of variance. Results fDPSCs were positive for STRO1, CD90, and CD105 and negative for CD146 markers with immunohistochemistry. fDPSCs showed strong osteogenic and weak adipogenic potential. The overall expression of DSPP was not significantly different between fDPSCs and human dental pulp cells. The expression of DSPP in osteo/odontogenic media was significantly higher in fDPSCs on day 4 (P <.01). The overall expression of dentin matrix protein-1, osteopontin, and alkaline phosphatase was significantly higher in fDPSCs (P =.0005). Conclusions fDPSCs were positive for several markers of dental pulp stem cells resembling human DPSCs and appeared to show a stronger potential to differentiate to osteoblastic rather than odontoblastic lineage.

Original languageEnglish (US)
Pages (from-to)418-424
Number of pages7
JournalJournal of endodontics
Volume42
Issue number3
DOIs
StatePublished - Mar 1 2016

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Dental Pulp
Ferrets
Stem Cells
Cuspid
Osteopontin
Dentin
Alkaline Phosphatase

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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Isolation, characterization, and differentiation of dental pulp stem cells in ferrets. / Homayounfar, Negar; Verma, Prashant; Nosrat, Ali; El Ayachi, Ikbale; Yu, Zongdong; Romberg, Elaine; Huang, George; Fouad, Ashraf F.

In: Journal of endodontics, Vol. 42, No. 3, 01.03.2016, p. 418-424.

Research output: Contribution to journalArticle

Homayounfar, Negar ; Verma, Prashant ; Nosrat, Ali ; El Ayachi, Ikbale ; Yu, Zongdong ; Romberg, Elaine ; Huang, George ; Fouad, Ashraf F. / Isolation, characterization, and differentiation of dental pulp stem cells in ferrets. In: Journal of endodontics. 2016 ; Vol. 42, No. 3. pp. 418-424.
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abstract = "Introduction The ferret canine tooth has been introduced as a suitable model for studying dental pulp regeneration. The aim of this study was to isolate and characterize ferret dental pulp stem cells (fDPSCs) and their differentiation potential. Methods Dental pulp stem cells were isolated from freshly extracted ferret canine teeth. The cells were examined for the expression of stem cell markers STRO-1, CD90, CD105, and CD146. The osteo/odontogenic and adipogenic differentiation potential of fDPSCs was evaluated. Osteogenic and odontogenic marker genes were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) on days 1, 4, and 8 after osteo/odontogenic induction of fDPSCs including dentin sialophosphoprotein (DSPP), dentin matrix protein-1, osteopontin, and alkaline phosphatase. Human dental pulp cells were used as the control. The results were analyzed using 3-way analysis of variance. Results fDPSCs were positive for STRO1, CD90, and CD105 and negative for CD146 markers with immunohistochemistry. fDPSCs showed strong osteogenic and weak adipogenic potential. The overall expression of DSPP was not significantly different between fDPSCs and human dental pulp cells. The expression of DSPP in osteo/odontogenic media was significantly higher in fDPSCs on day 4 (P <.01). The overall expression of dentin matrix protein-1, osteopontin, and alkaline phosphatase was significantly higher in fDPSCs (P =.0005). Conclusions fDPSCs were positive for several markers of dental pulp stem cells resembling human DPSCs and appeared to show a stronger potential to differentiate to osteoblastic rather than odontoblastic lineage.",
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AU - Romberg, Elaine

AU - Huang, George

AU - Fouad, Ashraf F.

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