Isolation, chromosomal mapping, and expression of the mouse tyrosinase gene

Byoung S. Kwon, Asifa K. Haq, Mark Wakulchik, Daniel Kestler, David E. Barton, Uta Francke, M. Lynn Lamoreux, J. Barry Whitney, Ruth Halaban

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Using a human tyrosinase cDNA probe, we have isolated mouse tyrosinase genomic clones and used them to map the mouse tyrosinase locus and to analyze the promoter sequence of the tyrosinase gene. Southern blot analyses of DNA from somatic cell hybrids, interspecies backcross mice, and albino deletion mice have revealed that the locus for mouse tyrosinase resides at or near the albino locus on mouse chromosome 7. There were three TATA-elements, but only one CAT-element, and the CAT-element appeared to be paired with the third TATA-element, located at the position farthest upstream. Mouse tyrosinase mRNA is approximately 2.4 Kb in size. The amount of tyrosinase mRNA reflects the levels of tyrosinase activity in normal melanocytes and Cloudman S-91 melanoma cell line.

Original languageEnglish (US)
Pages (from-to)589-594
Number of pages6
JournalJournal of Investigative Dermatology
Volume93
Issue number5
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

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Monophenol Monooxygenase
Genes
Messenger RNA
Chromosomes, Human, Pair 7
Hybrid Cells
Melanocytes
Chromosomes
Southern Blotting
Sequence Analysis
Melanoma
Complementary DNA
Clone Cells
Cells
Cell Line
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Kwon, B. S., Haq, A. K., Wakulchik, M., Kestler, D., Barton, D. E., Francke, U., ... Halaban, R. (1989). Isolation, chromosomal mapping, and expression of the mouse tyrosinase gene. Journal of Investigative Dermatology, 93(5), 589-594. https://doi.org/10.1111/1523-1747.ep12319693

Isolation, chromosomal mapping, and expression of the mouse tyrosinase gene. / Kwon, Byoung S.; Haq, Asifa K.; Wakulchik, Mark; Kestler, Daniel; Barton, David E.; Francke, Uta; Lamoreux, M. Lynn; Whitney, J. Barry; Halaban, Ruth.

In: Journal of Investigative Dermatology, Vol. 93, No. 5, 01.01.1989, p. 589-594.

Research output: Contribution to journalArticle

Kwon, BS, Haq, AK, Wakulchik, M, Kestler, D, Barton, DE, Francke, U, Lamoreux, ML, Whitney, JB & Halaban, R 1989, 'Isolation, chromosomal mapping, and expression of the mouse tyrosinase gene', Journal of Investigative Dermatology, vol. 93, no. 5, pp. 589-594. https://doi.org/10.1111/1523-1747.ep12319693
Kwon, Byoung S. ; Haq, Asifa K. ; Wakulchik, Mark ; Kestler, Daniel ; Barton, David E. ; Francke, Uta ; Lamoreux, M. Lynn ; Whitney, J. Barry ; Halaban, Ruth. / Isolation, chromosomal mapping, and expression of the mouse tyrosinase gene. In: Journal of Investigative Dermatology. 1989 ; Vol. 93, No. 5. pp. 589-594.
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