Isolation of purified and live Foxp3+ regulatory T cells using facs sorting on scatter plot

Xiaohui Zhou, Julie Wang, Wei Shi, David Brand, Zhongmin Liu, Huimin Fan, Song Guo Zheng

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

There are no ideal ways to identify and isolate viable and purified Foxp3+ regulatory T cells so far. Here we developed a novel procedure for the isolation of highly purified Foxp3+ cells using flow cytometry. This method relies on an identification and sorting of the lymphoblast cell population identified on a scatter plot using flow cytometry. We confirmed that greater than 98% of the cells sorted using this technique expressed Foxp3 and displayed a potent suppressive activity. This method provides a valuable tool for the study of the T regulatory cell biology and their therapeutic manipulation.

Original languageEnglish (US)
Pages (from-to)164-169
Number of pages6
JournalJournal of Molecular Cell Biology
Volume2
Issue number3
DOIs
StatePublished - Dec 1 2010
Externally publishedYes

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Regulatory T-Lymphocytes
Flow Cytometry
Cell Biology
Population
Therapeutics

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Isolation of purified and live Foxp3+ regulatory T cells using facs sorting on scatter plot. / Zhou, Xiaohui; Wang, Julie; Shi, Wei; Brand, David; Liu, Zhongmin; Fan, Huimin; Zheng, Song Guo.

In: Journal of Molecular Cell Biology, Vol. 2, No. 3, 01.12.2010, p. 164-169.

Research output: Contribution to journalArticle

Zhou, Xiaohui ; Wang, Julie ; Shi, Wei ; Brand, David ; Liu, Zhongmin ; Fan, Huimin ; Zheng, Song Guo. / Isolation of purified and live Foxp3+ regulatory T cells using facs sorting on scatter plot. In: Journal of Molecular Cell Biology. 2010 ; Vol. 2, No. 3. pp. 164-169.
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