Isolation of spectrin subunits by reverse-phase high-performance liquid chromatography

David G. Kakhniashvili, Steven Goodman

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We present a one-step uncomplicated method of separation of spectrin subunits. The method is based on reverse-phase HPLC employing an analytical C4 column. Reverse-phase HPLC combines the steps of dissociation and separation of spectrin subunits. The method can be applied to different spectrin isoforms. It can be used for analytical purposes, as well as for small-scale (<0.4 mg) isolation of spectrin subunits.

Original languageEnglish (US)
Pages (from-to)249-251
Number of pages3
JournalProtein Expression and Purification
Volume23
Issue number2
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

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Spectrin
Reverse-Phase Chromatography
High Pressure Liquid Chromatography
Protein Isoforms

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

Isolation of spectrin subunits by reverse-phase high-performance liquid chromatography. / Kakhniashvili, David G.; Goodman, Steven.

In: Protein Expression and Purification, Vol. 23, No. 2, 01.01.2001, p. 249-251.

Research output: Contribution to journalArticle

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