Karyometry of nuclear phenotypes in cutaneous squamous cell cancer

Peter H. Bartels, Hubert G. Bartels, David S. Alberts, Michael Yozwiak, Anil R. Prasad, Evan Glazer, Robert S. Krouse

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: To establish the karyometric characteristics of the two main nuclear phenotypes in cutaneous squamous cell cancer (cSCC) lesions. Study Design: The clinical materials comprised 75 cases of cSCC, 38 with aggressive lesions and 37 with nonaggressive lesions. High-resolution images of 100 nuclei per case were recorded. Data were partitioned into four subgroups covering the range of lesion progression. Four discriminant functions were derived to distinguish aggressive from nonaggressive lesions. The most typical nuclei from the phenotype predominant in aggressive lesions and nonaggressive lesions were separated out by thresholding on the discriminant function score axes. For these homogeneous sets of nuclei the karyometric features were computed. Results: The nuclear populations in cSCC lesions are a very heterogeneous set. There are two axes of dispersion, along the line of lesion progression and between aggressive and nonaggressive lesions. The analysis faces the difficulty that lesions from both diagnostic categories contain nuclei of the same two phenotypes with the difference between categories consisting only of differences in proportion of the two phenotypes. Conclusion: The nuclei of the aggressive phenotype I and nonaggressive phenotype II have substantially different chromatin patterns and can be distinguished with > 90% correct recognition rate.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalAnalytical and Quantitative Cytology and Histology
Volume34
Issue number1
StatePublished - Feb 1 2012

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Karyometry
Squamous Cell Neoplasms
Skin Neoplasms
Phenotype
Chromatin

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

Cite this

Bartels, P. H., Bartels, H. G., Alberts, D. S., Yozwiak, M., Prasad, A. R., Glazer, E., & Krouse, R. S. (2012). Karyometry of nuclear phenotypes in cutaneous squamous cell cancer. Analytical and Quantitative Cytology and Histology, 34(1), 1-8.

Karyometry of nuclear phenotypes in cutaneous squamous cell cancer. / Bartels, Peter H.; Bartels, Hubert G.; Alberts, David S.; Yozwiak, Michael; Prasad, Anil R.; Glazer, Evan; Krouse, Robert S.

In: Analytical and Quantitative Cytology and Histology, Vol. 34, No. 1, 01.02.2012, p. 1-8.

Research output: Contribution to journalArticle

Bartels, PH, Bartels, HG, Alberts, DS, Yozwiak, M, Prasad, AR, Glazer, E & Krouse, RS 2012, 'Karyometry of nuclear phenotypes in cutaneous squamous cell cancer', Analytical and Quantitative Cytology and Histology, vol. 34, no. 1, pp. 1-8.
Bartels PH, Bartels HG, Alberts DS, Yozwiak M, Prasad AR, Glazer E et al. Karyometry of nuclear phenotypes in cutaneous squamous cell cancer. Analytical and Quantitative Cytology and Histology. 2012 Feb 1;34(1):1-8.
Bartels, Peter H. ; Bartels, Hubert G. ; Alberts, David S. ; Yozwiak, Michael ; Prasad, Anil R. ; Glazer, Evan ; Krouse, Robert S. / Karyometry of nuclear phenotypes in cutaneous squamous cell cancer. In: Analytical and Quantitative Cytology and Histology. 2012 ; Vol. 34, No. 1. pp. 1-8.
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