KLF4 promotes angiogenesis by activating VEGF signaling in human retinal microvascular endothelial cells

Yinan Wang, Chuan Yang, Qingqing Gu, Michelle Sims, Weiwang Gu, Lawrence Pfeffer, Junming Yue

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The transcription factor Krüppel-like factor 4 (KLF4) has been implicated in regulating cell proliferation, migration and differentiation in a variety of human cells and is one of four factors required for the induction of pluripotent stem cell reprogramming. However, its role has not been addressed in ocular neovascular diseases. This study investigated the role of KLF4 in angiogenesis and underlying molecular mechanisms in human retinal microvascular endothelial cells (HRMECs). The functional role of KLF4 in HRMECs was determined following lentiviral vector mediated inducible expression and shRNA knockdown of KLF4. Inducible expression of KLF4 promotes cell proliferation, migration and tube formation. In contrast, silencing KLF4 inhibits cell proliferation, migration, tube formation and induces apoptosis in HRMECs. KLF4 promotes angiogenesis by transcriptionally activating VEGF expression, thus activating the VEGF signaling pathway in HRMECs.

Original languageEnglish (US)
Article numbere0130341
JournalPLoS ONE
Volume10
Issue number6
DOIs
StatePublished - Jun 15 2015

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Endothelial cells
angiogenesis
Vascular Endothelial Growth Factor A
endothelial cells
Cell proliferation
Endothelial Cells
Cell Movement
cell proliferation
Cell Proliferation
eye diseases
Stem cells
Pluripotent Stem Cells
Eye Diseases
Small Interfering RNA
Transcription Factors
Cells
stem cells
Apoptosis
Cell Differentiation
apoptosis

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

KLF4 promotes angiogenesis by activating VEGF signaling in human retinal microvascular endothelial cells. / Wang, Yinan; Yang, Chuan; Gu, Qingqing; Sims, Michelle; Gu, Weiwang; Pfeffer, Lawrence; Yue, Junming.

In: PLoS ONE, Vol. 10, No. 6, e0130341, 15.06.2015.

Research output: Contribution to journalArticle

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AU - Yue, Junming

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