Kymographic Imaging of the Elastic Modulus of Epithelial Cells during the Onset of Migration

Esra Roan, Kristina R. Wilhelm, Christopher Waters

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Epithelial cell migration during wound repair involves a complex interplay of intracellular processes that enable motility while preserving contact among the cells. Recent evidence suggests that fluctuations of the intracellular biophysical state of cells generate traction forces at the basal side of the cells that are necessary for the cells to migrate. However, less is known about the biophysical and structural changes throughout the cells that accompany these fluctuations. Here, we utilized, to our knowledge, a novel kymographic nanoindentation method to obtain spatiotemporal measurements of the elastic moduli of living cells during migration after wounding. At the onset of migration, the elastic modulus increased near the migration front. In addition, the intensity of fluctuations in the elastic modulus changed at the migration front, and these changes were dependent upon f-actin, one of the major components of the cytoskeleton. These results demonstrate the unique biophysical changes that occur at the onset of migration as cells transition from a stationary to a migratory state.

Original languageEnglish (US)
Pages (from-to)2051-2057
Number of pages7
JournalBiophysical Journal
Volume109
Issue number10
DOIs
StatePublished - Nov 17 2015

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Elastic Modulus
Epithelial Cells
Cell Movement
Traction
Cytoskeleton
Actins
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

Kymographic Imaging of the Elastic Modulus of Epithelial Cells during the Onset of Migration. / Roan, Esra; Wilhelm, Kristina R.; Waters, Christopher.

In: Biophysical Journal, Vol. 109, No. 10, 17.11.2015, p. 2051-2057.

Research output: Contribution to journalArticle

Roan, Esra ; Wilhelm, Kristina R. ; Waters, Christopher. / Kymographic Imaging of the Elastic Modulus of Epithelial Cells during the Onset of Migration. In: Biophysical Journal. 2015 ; Vol. 109, No. 10. pp. 2051-2057.
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