Lactulose feeding lowers cecal densities of clostridia in piglets

C. Lawrence Kien, Ruth Blauwiekel, Carol H. Williams, Janice Yanushka Bunn, Randal Buddington

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: In order to understand the consequences of persistent enteral feeding in patients with carbohydrate malabsorption, we fed piglets lactulose in sufficient dosage to produce osmotic diarrhea or inulin, using a conventional dose, to determine if this prebiotic can modulate the effects of lactulose. Feeding lactulose increases cecal luminal synthesis of butyrate, with inulin having an intermediate effect. Because clostridia may be a major source of colonic butyrate production, we hypothesized that feeding piglets lactulose or inulin would increase cecal densities of clostridia. Methods: Piglets were assigned to 3 formula study groups for 6 days: (1) control, fed only sow milk replacer (n = 12); (2) inulin, inulin supplement (3 g/L; n = 11); and (3) lactulose, lactulose supplement (66.7 g/L; n = 6). Cecal fluid for bacteriological studies was sampled intraoperatively. Results: The wet/dry ratio of the cecal contents (mean ± SEM) was 8.2 ± 0.5, 6.2 ± 0.5, and 18.8 ± 5.5, respectively, in the control, inulin, and lactulose groups (p = .049, Kruskal-Wallis). There were no differences among the diet groups for cecal densities (106 colony-forming units [CFU]/g dry wt cecal contents) of total anaerobes, total aerobes, bifidobacteria, or lactobacilli. Densities of clostridia were markedly reduced in the lactulose group (1.14 ± 0.41) vs the control (18.39 ± 4.44; p = .001) or inulin groups (8.87 ± 2.20; p = .04). Conclusions: In piglets, feeding lactulose at a dose known to cause diarrhea reduces cecal densities of clostridia.

Original languageEnglish (US)
Pages (from-to)194-198
Number of pages5
JournalJournal of Parenteral and Enteral Nutrition
Volume31
Issue number3
DOIs
StatePublished - May 1 2007

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Lactulose
Clostridium
Inulin
Butyrates
Diarrhea
Prebiotics
Bifidobacterium
Enteral Nutrition
Lactobacillus
Milk
Stem Cells
Carbohydrates
Diet

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Lactulose feeding lowers cecal densities of clostridia in piglets. / Kien, C. Lawrence; Blauwiekel, Ruth; Williams, Carol H.; Bunn, Janice Yanushka; Buddington, Randal.

In: Journal of Parenteral and Enteral Nutrition, Vol. 31, No. 3, 01.05.2007, p. 194-198.

Research output: Contribution to journalArticle

Kien, C. Lawrence ; Blauwiekel, Ruth ; Williams, Carol H. ; Bunn, Janice Yanushka ; Buddington, Randal. / Lactulose feeding lowers cecal densities of clostridia in piglets. In: Journal of Parenteral and Enteral Nutrition. 2007 ; Vol. 31, No. 3. pp. 194-198.
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abstract = "Background: In order to understand the consequences of persistent enteral feeding in patients with carbohydrate malabsorption, we fed piglets lactulose in sufficient dosage to produce osmotic diarrhea or inulin, using a conventional dose, to determine if this prebiotic can modulate the effects of lactulose. Feeding lactulose increases cecal luminal synthesis of butyrate, with inulin having an intermediate effect. Because clostridia may be a major source of colonic butyrate production, we hypothesized that feeding piglets lactulose or inulin would increase cecal densities of clostridia. Methods: Piglets were assigned to 3 formula study groups for 6 days: (1) control, fed only sow milk replacer (n = 12); (2) inulin, inulin supplement (3 g/L; n = 11); and (3) lactulose, lactulose supplement (66.7 g/L; n = 6). Cecal fluid for bacteriological studies was sampled intraoperatively. Results: The wet/dry ratio of the cecal contents (mean ± SEM) was 8.2 ± 0.5, 6.2 ± 0.5, and 18.8 ± 5.5, respectively, in the control, inulin, and lactulose groups (p = .049, Kruskal-Wallis). There were no differences among the diet groups for cecal densities (106 colony-forming units [CFU]/g dry wt cecal contents) of total anaerobes, total aerobes, bifidobacteria, or lactobacilli. Densities of clostridia were markedly reduced in the lactulose group (1.14 ± 0.41) vs the control (18.39 ± 4.44; p = .001) or inulin groups (8.87 ± 2.20; p = .04). Conclusions: In piglets, feeding lactulose at a dose known to cause diarrhea reduces cecal densities of clostridia.",
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