Laser-capture microdissection of hyperlipidemic/ApoE -/- mouse aorta atherosclerosis

Michael Beer, Sandra Doepping, Markus Hildner, Gabriele Weber, Rolf Grabner, Desheng Hu, Sarajo Kumar Mohanta, Prasad Srikakulapu, Falk Weih, Andreas J.R. Habenicht

Research output: Chapter in Book/Report/Conference proceedingChapter

15 Citations (Scopus)

Abstract

Atherosclerosis is a transmural chronic inflammatory condition of small and large arteries that is associated with adaptive immune responses at all disease stages. However, impacts of adaptive immune reactions on clinically apparent atherosclerosis such as intima lesion (plaque) rupture, thrombosis, myocardial infar-ction, and aneurysm largely remain to be identified. It is increasingly recognized that leukocyte infiltrates in plaque, media, and adventitia are distinct but that their specific roles have not been defined. To map these infiltrates, we employed laser-capture microdissection (LCM) to isolate the three arterial wall laminae using apoE -/- mouse aorta as a model. RNA from LCM-separated tissues was extracted and large-scale, whole-genome expression microarrays were prepared. We observed that the quality of the resulting gene expression maps was compromised by tissue RNA carried over from adjacent laminae during LCM. To account for these flaws, we established quality controls and algorithms to improve the predictive power of LCM-derived microarray data. Our approach creates robust transcriptome atlases of normal and atherosclerotic aorta. Assessing LCM transcriptomes for immunity-related mRNAs indicated markedly distinctive gene expression patterns in the three laminae of the atherosclerotic aorta. These mouse mRNA expression data banks can now be mined to address a wide range of questions in cardiovascular biology.

Original languageEnglish (US)
Title of host publicationLaser Capture Microdissection
Subtitle of host publicationMethods and Protocols
EditorsGraeme I. Murray
Pages417-428
Number of pages12
DOIs
StatePublished - Aug 4 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume755
ISSN (Print)1064-3745

Fingerprint

Laser Capture Microdissection
Apolipoproteins E
Aorta
Atherosclerosis
Transcriptome
RNA
Gene Expression
Adventitia
Messenger RNA
Atlases
Adaptive Immunity
Quality Control
Aneurysm
Rupture
Immunity
Thrombosis
Leukocytes
Arteries
Genome
Databases

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Beer, M., Doepping, S., Hildner, M., Weber, G., Grabner, R., Hu, D., ... Habenicht, A. J. R. (2011). Laser-capture microdissection of hyperlipidemic/ApoE -/- mouse aorta atherosclerosis. In G. I. Murray (Ed.), Laser Capture Microdissection: Methods and Protocols (pp. 417-428). (Methods in Molecular Biology; Vol. 755). https://doi.org/10.1007/978-1-61779-163-5_35

Laser-capture microdissection of hyperlipidemic/ApoE -/- mouse aorta atherosclerosis. / Beer, Michael; Doepping, Sandra; Hildner, Markus; Weber, Gabriele; Grabner, Rolf; Hu, Desheng; Mohanta, Sarajo Kumar; Srikakulapu, Prasad; Weih, Falk; Habenicht, Andreas J.R.

Laser Capture Microdissection: Methods and Protocols. ed. / Graeme I. Murray. 2011. p. 417-428 (Methods in Molecular Biology; Vol. 755).

Research output: Chapter in Book/Report/Conference proceedingChapter

Beer, M, Doepping, S, Hildner, M, Weber, G, Grabner, R, Hu, D, Mohanta, SK, Srikakulapu, P, Weih, F & Habenicht, AJR 2011, Laser-capture microdissection of hyperlipidemic/ApoE -/- mouse aorta atherosclerosis. in GI Murray (ed.), Laser Capture Microdissection: Methods and Protocols. Methods in Molecular Biology, vol. 755, pp. 417-428. https://doi.org/10.1007/978-1-61779-163-5_35
Beer M, Doepping S, Hildner M, Weber G, Grabner R, Hu D et al. Laser-capture microdissection of hyperlipidemic/ApoE -/- mouse aorta atherosclerosis. In Murray GI, editor, Laser Capture Microdissection: Methods and Protocols. 2011. p. 417-428. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-163-5_35
Beer, Michael ; Doepping, Sandra ; Hildner, Markus ; Weber, Gabriele ; Grabner, Rolf ; Hu, Desheng ; Mohanta, Sarajo Kumar ; Srikakulapu, Prasad ; Weih, Falk ; Habenicht, Andreas J.R. / Laser-capture microdissection of hyperlipidemic/ApoE -/- mouse aorta atherosclerosis. Laser Capture Microdissection: Methods and Protocols. editor / Graeme I. Murray. 2011. pp. 417-428 (Methods in Molecular Biology).
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