Lateral hypothalamus and local stimulation induced postsynaptic responses in zona incerta neurons in an in vitro slice preparation of the rat

Hiroshi Nakanishi, Kenji Yamamoto, Hitoshi Kita

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Postsynaptic potentials evoked in the zona incerta (ZI) neurons were studied in in vitro slice preparations. Lateral hypothalamus (LH) and local stimulation evoked fast IPSPs, fast EPSPs, and slow EPSPs. The amplitude of the slow EPSPs increased when the neuron was hyperpolarized by a low intensity current injection but was blocked when it was hyperpolarized with a strong current. The slow EPSPs were reversibly suppressed by an application of 50 μM DL-2-amino-5-phosphonovaleric acid (APV) and 20 μM 3-[(±)-2-carboxypiperazine-4-yl-]-propyl-1-phosphonic acid (CPP). The slow EPSPs were augmented in Mg-free medium and by train pulse stimulation. Pressure application of NMDA induced a depolarization similar to the slow EPSP. On the other hand, the fast EPSPs showed a conventional voltage dependency and were antagonized by kynurenic acid but not by APV or CPP. The fast IPSPs were completely blocked by 10 μM bicuculline methiodide. The results indicate that LH and local stimulation evoked monosynaptic fast EPSPs and slow EPSPs mediated by N-methyl-D-aspartate (NMDA) and non-NMDA receptors, respectively. The IPSPs appear to be mediated by GABAA receptors and regulate the expression of NMDA receptor-mediated slow EPSPs.

Original languageEnglish (US)
Pages (from-to)287-291
Number of pages5
JournalBrain Research
Volume583
Issue number1-2
DOIs
StatePublished - Jan 1 1992

Fingerprint

Lateral Hypothalamic Area
Excitatory Postsynaptic Potentials
Neurons
Inhibitory Postsynaptic Potentials
N-Methylaspartate
Zona Incerta
In Vitro Techniques
Kynurenic Acid
2-Amino-5-phosphonovalerate
D-Aspartic Acid
Synaptic Potentials
GABA-A Receptors
N-Methyl-D-Aspartate Receptors

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Lateral hypothalamus and local stimulation induced postsynaptic responses in zona incerta neurons in an in vitro slice preparation of the rat. / Nakanishi, Hiroshi; Yamamoto, Kenji; Kita, Hitoshi.

In: Brain Research, Vol. 583, No. 1-2, 01.01.1992, p. 287-291.

Research output: Contribution to journalArticle

@article{50489b3b758f434ba2fff1ab54673805,
title = "Lateral hypothalamus and local stimulation induced postsynaptic responses in zona incerta neurons in an in vitro slice preparation of the rat",
abstract = "Postsynaptic potentials evoked in the zona incerta (ZI) neurons were studied in in vitro slice preparations. Lateral hypothalamus (LH) and local stimulation evoked fast IPSPs, fast EPSPs, and slow EPSPs. The amplitude of the slow EPSPs increased when the neuron was hyperpolarized by a low intensity current injection but was blocked when it was hyperpolarized with a strong current. The slow EPSPs were reversibly suppressed by an application of 50 μM DL-2-amino-5-phosphonovaleric acid (APV) and 20 μM 3-[(±)-2-carboxypiperazine-4-yl-]-propyl-1-phosphonic acid (CPP). The slow EPSPs were augmented in Mg-free medium and by train pulse stimulation. Pressure application of NMDA induced a depolarization similar to the slow EPSP. On the other hand, the fast EPSPs showed a conventional voltage dependency and were antagonized by kynurenic acid but not by APV or CPP. The fast IPSPs were completely blocked by 10 μM bicuculline methiodide. The results indicate that LH and local stimulation evoked monosynaptic fast EPSPs and slow EPSPs mediated by N-methyl-D-aspartate (NMDA) and non-NMDA receptors, respectively. The IPSPs appear to be mediated by GABAA receptors and regulate the expression of NMDA receptor-mediated slow EPSPs.",
author = "Hiroshi Nakanishi and Kenji Yamamoto and Hitoshi Kita",
year = "1992",
month = "1",
day = "1",
doi = "10.1016/S0006-8993(10)80035-6",
language = "English (US)",
volume = "583",
pages = "287--291",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Lateral hypothalamus and local stimulation induced postsynaptic responses in zona incerta neurons in an in vitro slice preparation of the rat

AU - Nakanishi, Hiroshi

AU - Yamamoto, Kenji

AU - Kita, Hitoshi

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Postsynaptic potentials evoked in the zona incerta (ZI) neurons were studied in in vitro slice preparations. Lateral hypothalamus (LH) and local stimulation evoked fast IPSPs, fast EPSPs, and slow EPSPs. The amplitude of the slow EPSPs increased when the neuron was hyperpolarized by a low intensity current injection but was blocked when it was hyperpolarized with a strong current. The slow EPSPs were reversibly suppressed by an application of 50 μM DL-2-amino-5-phosphonovaleric acid (APV) and 20 μM 3-[(±)-2-carboxypiperazine-4-yl-]-propyl-1-phosphonic acid (CPP). The slow EPSPs were augmented in Mg-free medium and by train pulse stimulation. Pressure application of NMDA induced a depolarization similar to the slow EPSP. On the other hand, the fast EPSPs showed a conventional voltage dependency and were antagonized by kynurenic acid but not by APV or CPP. The fast IPSPs were completely blocked by 10 μM bicuculline methiodide. The results indicate that LH and local stimulation evoked monosynaptic fast EPSPs and slow EPSPs mediated by N-methyl-D-aspartate (NMDA) and non-NMDA receptors, respectively. The IPSPs appear to be mediated by GABAA receptors and regulate the expression of NMDA receptor-mediated slow EPSPs.

AB - Postsynaptic potentials evoked in the zona incerta (ZI) neurons were studied in in vitro slice preparations. Lateral hypothalamus (LH) and local stimulation evoked fast IPSPs, fast EPSPs, and slow EPSPs. The amplitude of the slow EPSPs increased when the neuron was hyperpolarized by a low intensity current injection but was blocked when it was hyperpolarized with a strong current. The slow EPSPs were reversibly suppressed by an application of 50 μM DL-2-amino-5-phosphonovaleric acid (APV) and 20 μM 3-[(±)-2-carboxypiperazine-4-yl-]-propyl-1-phosphonic acid (CPP). The slow EPSPs were augmented in Mg-free medium and by train pulse stimulation. Pressure application of NMDA induced a depolarization similar to the slow EPSP. On the other hand, the fast EPSPs showed a conventional voltage dependency and were antagonized by kynurenic acid but not by APV or CPP. The fast IPSPs were completely blocked by 10 μM bicuculline methiodide. The results indicate that LH and local stimulation evoked monosynaptic fast EPSPs and slow EPSPs mediated by N-methyl-D-aspartate (NMDA) and non-NMDA receptors, respectively. The IPSPs appear to be mediated by GABAA receptors and regulate the expression of NMDA receptor-mediated slow EPSPs.

UR - http://www.scopus.com/inward/record.url?scp=0026654407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026654407&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(10)80035-6

DO - 10.1016/S0006-8993(10)80035-6

M3 - Article

VL - 583

SP - 287

EP - 291

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -