LC-MS/MS determination of bakkenolide D in rats plasma and its application in pharmacokinetic studies

Dongmei Dai, Lixia Pei, Lin Tang, Feng Chen, Xiuping Chen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for determination of bakkenolide D (BD), which was further applied to assess the pharmacokinetics of BD. In the LC-MS/MS method, the multiple reaction monitoring mode was used and columbianadin was chosen as internal standard. The method was validated over the range of 1-800 ng/mL with a determination coefficient >0.999. The lower limit of quantification was 1 ng/mL in plasma. The intra- and inter-day accuracies for BD were 91-113 and 100-104%, respectively, and the inter-day precision was <15%. After a single oral dose of 10 mg/kg of BD, the mean peak plasma concentration of BD was 10.1±9.8 ng/mL at 2 h. The area under the plasma concentration-time curve (AUC0-24 h) was 72.1±8.59 h ng/mL, and the elimination half-life (T1/2) was 11.8±1.9 h. In case of intravenous administration of BD at a dosage of 1 mg/kg, the AUC0-24 h was 281±98.4 h{dot operator}ng/mL, and the T1/2 was 8.79±0.63 h. Based on these results, the oral bioavailability of BD in rats at 10 mg/kg is 2.57%.

Original languageEnglish (US)
Pages (from-to)1200-1207
Number of pages8
JournalBiomedical Chromatography
Volume27
Issue number9
DOIs
StatePublished - Jan 1 2013

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Pharmacokinetics
Rats
Plasmas
Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Intravenous Administration
Biological Availability
Mass spectrometry
Half-Life
Monitoring

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

LC-MS/MS determination of bakkenolide D in rats plasma and its application in pharmacokinetic studies. / Dai, Dongmei; Pei, Lixia; Tang, Lin; Chen, Feng; Chen, Xiuping.

In: Biomedical Chromatography, Vol. 27, No. 9, 01.01.2013, p. 1200-1207.

Research output: Contribution to journalArticle

Dai, Dongmei ; Pei, Lixia ; Tang, Lin ; Chen, Feng ; Chen, Xiuping. / LC-MS/MS determination of bakkenolide D in rats plasma and its application in pharmacokinetic studies. In: Biomedical Chromatography. 2013 ; Vol. 27, No. 9. pp. 1200-1207.
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