LC-MS/MS identification of the one-carbon cycle metabolites in human plasma

Lidia A. Gardner, Dominic M. Desiderio, Chassidy J. Groover, Anastasia Hartzes, Charles Yates, Audrey Zucker-Levin, Leonard Bloom, Michael C. Levin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The one-carbon cycle is composed of four major biologically important molecules: methionine (l-Met), S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), and homocysteine (Hcy). In addition to these key metabolites, there are multiple enzymes, vitamins, and cofactors that play essential roles in the cascade of the biochemical reactions that convert one metabolite into another in the cycle. Simultaneous quantitative measurement of four major metabolites can be used to detect possible aberrations in this vital cycle. Abnormalities in the one-carbon cycle might lead to hyper- or hypomethylation, homocystinemia, liver dysfunction, and accumulation of white-matter hyperintensities in the human brain. Previously published methods describe evaluation of several components of the one-carbon cycle, but none to our knowledge demonstrated simultaneous measurement of all four key molecules (l-Met, SAM, SAH, and Hcy). We describe a novel analytical method suitable for simultaneous identification and quantification of l-Met, SAM, SAH, and Hcy with LC-MS/MS. Moreover, we tested this method to identify these metabolites in human plasma collected from patients with multiple sclerosis and healthy individuals. In a pilot feasibility study, our results indicate that patients with multiple sclerosis showed abnormalities in the one-carbon cycle.

Original languageEnglish (US)
Pages (from-to)1710-1716
Number of pages7
JournalElectrophoresis
Volume34
Issue number11
DOIs
StatePublished - Jun 1 2013

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Plasma (human)
Carbon Cycle
S-Adenosylhomocysteine
Metabolites
S-Adenosylmethionine
Homocysteine
Carbon
Multiple Sclerosis
Molecules
Coenzymes
Feasibility Studies
Aberrations
Vitamins
Methionine
Liver
Liver Diseases
Brain
Enzymes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

Gardner, L. A., Desiderio, D. M., Groover, C. J., Hartzes, A., Yates, C., Zucker-Levin, A., ... Levin, M. C. (2013). LC-MS/MS identification of the one-carbon cycle metabolites in human plasma. Electrophoresis, 34(11), 1710-1716. https://doi.org/10.1002/elps.201200536

LC-MS/MS identification of the one-carbon cycle metabolites in human plasma. / Gardner, Lidia A.; Desiderio, Dominic M.; Groover, Chassidy J.; Hartzes, Anastasia; Yates, Charles; Zucker-Levin, Audrey; Bloom, Leonard; Levin, Michael C.

In: Electrophoresis, Vol. 34, No. 11, 01.06.2013, p. 1710-1716.

Research output: Contribution to journalArticle

Gardner, LA, Desiderio, DM, Groover, CJ, Hartzes, A, Yates, C, Zucker-Levin, A, Bloom, L & Levin, MC 2013, 'LC-MS/MS identification of the one-carbon cycle metabolites in human plasma', Electrophoresis, vol. 34, no. 11, pp. 1710-1716. https://doi.org/10.1002/elps.201200536
Gardner, Lidia A. ; Desiderio, Dominic M. ; Groover, Chassidy J. ; Hartzes, Anastasia ; Yates, Charles ; Zucker-Levin, Audrey ; Bloom, Leonard ; Levin, Michael C. / LC-MS/MS identification of the one-carbon cycle metabolites in human plasma. In: Electrophoresis. 2013 ; Vol. 34, No. 11. pp. 1710-1716.
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