Lentiviral vector mediated-ASAP1 expression promotes epithelial to mesenchymal transition in ovarian cancer cells

Tao Zhang, Guannan Zhao, Chuan Yang, Peixin Dong, Hidemichi Watari, Lin Zeng, Lawrence Pfeffer, Junming Yue

Research output: Contribution to journalArticle

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Abstract

Ovarian cancer is one of the most common malignancies in women and has a high mortality rate due to metastatic progression and tumor recurrence. ASAP1 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 1) is an ADP-ribosylation factor GTPase-activating protein, which is involved in tumor metastasis. However, the role of ASAP1 in ovarian cancer is completely unknown. The present study reported that ASAP1 was highly expressed in ovarian carcinoma, and expression positively-correlated with overall poor survival and prognosis of patients. Lentiviral vector mediated ASAP1 expression promoted cell migration and invasion in ovarian cancer cell lines SKOV3 and OVCAR3. In addition, ASAP1 promoted cell proliferation, survival and inhibited chemotherapy drug paclitaxel-induced cell apoptosis. Furthermore, ASAP1 expression promoted epithelial to mesenchymal transition (EMT) by upregulating the mesenchymal cell markers N-cadherin and vimentin, and downregulating epithelial cell marker E-cadherin in the ovarian cancer cell lines. The data indicate for the first time that ASAP1 exhibits an oncogenic role by promoting EMT in ovarian cancer cells.

Original languageEnglish (US)
Pages (from-to)4432-4438
Number of pages7
JournalOncology Letters
Volume15
Issue number4
DOIs
StatePublished - Apr 1 2018

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Epithelial-Mesenchymal Transition
Ovarian Neoplasms
Cadherins
ADP-Ribosylation Factor 1
Ankyrin Repeat
GTPase-Activating Proteins
Cell Line
Neoplasms
src Homology Domains
Vimentin
Paclitaxel
Cell Movement
Cell Survival
Down-Regulation
Epithelial Cells
Cell Proliferation
Apoptosis
Neoplasm Metastasis
Carcinoma
Recurrence

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Lentiviral vector mediated-ASAP1 expression promotes epithelial to mesenchymal transition in ovarian cancer cells. / Zhang, Tao; Zhao, Guannan; Yang, Chuan; Dong, Peixin; Watari, Hidemichi; Zeng, Lin; Pfeffer, Lawrence; Yue, Junming.

In: Oncology Letters, Vol. 15, No. 4, 01.04.2018, p. 4432-4438.

Research output: Contribution to journalArticle

Zhang, Tao ; Zhao, Guannan ; Yang, Chuan ; Dong, Peixin ; Watari, Hidemichi ; Zeng, Lin ; Pfeffer, Lawrence ; Yue, Junming. / Lentiviral vector mediated-ASAP1 expression promotes epithelial to mesenchymal transition in ovarian cancer cells. In: Oncology Letters. 2018 ; Vol. 15, No. 4. pp. 4432-4438.
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