Leukotriene C4

The major lipoxygenase metabolite of arachidonic acid in dog spleen

Kafait Malik, Patrick Y.K. Wong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Slices of dog spleen converted [14C]-arachidonic acid (AA) to a polar material which conjugated with [3H]-glutatione. Nordihydroguaiaretic acid (NDGA) and 5,8,11,14, Eicosatetraynoic acid (ETYA) but not indomethacin, inhibited the conversion of [14C]-arachidonic acid by the spleen slices into the polar material indicating that it is derived through the lipoxygenase pathway. Physicochemical analysis of the polar metabolite of arachidonic acid after thin-layer chromatography and high pressure liquid chromatography revealed that it has chemical properties identical to authentic leukotriene C4 standard (LTC4). The biological activity of the purified material was found to be similar to the slow reacting substance of anaphylaxis (SRS-A), viz, it caused contraction of the guinea-pig ileum which was abolished by FPL-55172, a specific SRS-A receptor antagonist. These data suggest that dog spleen slices convert arachidonic acid through lipoxygenase pathway into a polar material that appears to be identical to LTC4.

Original languageEnglish (US)
Pages (from-to)511-520
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume103
Issue number2
DOIs
StatePublished - Nov 30 1981

Fingerprint

Arachidonate Lipoxygenases
Leukotriene C4
Lipoxygenase
Metabolites
Arachidonic Acid
Spleen
Dogs
5,8,11,14-Eicosatetraynoic Acid
SRS-A
Leukotriene Receptors
Masoprocol
High pressure liquid chromatography
Thin Layer Chromatography
Ileum
Indomethacin
Thin layer chromatography
Guinea Pigs
Bioactivity
High Pressure Liquid Chromatography
Chemical properties

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Leukotriene C4 : The major lipoxygenase metabolite of arachidonic acid in dog spleen. / Malik, Kafait; Wong, Patrick Y.K.

In: Biochemical and Biophysical Research Communications, Vol. 103, No. 2, 30.11.1981, p. 511-520.

Research output: Contribution to journalArticle

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