Leukotrienes increase levels of prostanoids in cerebrospinal fluid in piglets

David W. Busija, Charles Leffler

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We investigated effects of exogenous leukotrienes (C4, D4, or E4 on levels of prostanoids in cerebrospinal fluid in newborn pigs (1-5 days). A "close" cranial window was placed over the parietal cortex. Pial arterial diameter was measured with a microscope and electronic micrometer system. Levels in cerebrospinal fluid (CSF) of 6-keto-Prostaglandin F (6-keto-PGF), Thromboxane B2 (TXB2), and Prostaglandin E2 (PGE2) were measured by radioimmunoassay. Topical application of leukotrienes C4, D4, or E4 (5,000 ng/ml) similarly constricted pial arteries by 15 ± 2% (n = 14) (mean ± SEM). In addition, leukotrienes increased levels of 6-keto-PGF from 806 ± 136 to 1,612 ± 304 pg/ml (n = 13), TXB2 from 161 ± 31 to 392 ± 81 pg/ml(n = 10), and PGE2 from 2,271 ± 342 to 4,636 ± 740 pg/ml (n = 13). Each type of leukotriene had similar effects on prostanoid synthesis. In other experiments (n = 5), we found that 0.2 ng/ml PGE2 in CSF dilated pial arteries by 24 ± 8% and that 1.0 ng/mg PGI2 dilated pial arterial by 15 ± 6%. These results indicate that leukotrienes are able to increase levels of prostanoids in cerebral cortex.

Original languageEnglish (US)
Pages (from-to)803-811
Number of pages9
JournalProstaglandins
Volume32
Issue number6
DOIs
StatePublished - Jan 1 1986

Fingerprint

Cerebrospinal fluid
Leukotrienes
Dinoprostone
Leukotriene E4
Prostaglandins
Leukotriene D4
Cerebrospinal Fluid
Leukotriene C4
Thromboxane B2
Arteries
Parietal Lobe
Epoprostenol
Cerebral Cortex
Radioimmunoassay
Microscopes
Swine
Scanning electron microscopy
Experiments
prostaglandin F1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology

Cite this

Leukotrienes increase levels of prostanoids in cerebrospinal fluid in piglets. / Busija, David W.; Leffler, Charles.

In: Prostaglandins, Vol. 32, No. 6, 01.01.1986, p. 803-811.

Research output: Contribution to journalArticle

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