Light chain amyloid fibrils cause metabolic dysfunction in human cardiomyocytes

Helen P. McWilliams-Koeppen, James S. Foster, Nicole Hackenbrack, Marina Ramirez-Alvarado, Dallas Donohoe, Angela Williams, Sallie Macy, Craig Wooliver, Dale Wortham, Jennifer Morrell-Falvey, Carmen M. Foster, Stephen Kennel, Jonathan Wall

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Light chain (AL) amyloidosis is the most common form of systemic amyloid disease, and cardiomyopathy is a dire consequence, resulting in an extremely poor prognosis. AL is characterized by the production of monoclonal free light chains that deposit as amyloid fibrils principally in the heart, liver, and kidneys causing organ dysfunction. We have studied the effects of amyloid fibrils, produced from recombinant γ6 light chain variable domains, on metabolic activity of human cardiomyocytes. The data indicate that fibrils at 0.1 μM, but not monomer, significantly decrease the enzymatic activity of cellular NAD(P)H-dependent oxidoreductase, without causing significant cell death. The presence of amyloid fibrils did not affect ATP levels; however, oxygen consumption was increased and reactive oxygen species were detected. Confocal fluorescence microscopy showed that fibrils bound to and remained at the cell surface with little fibril internalization. These data indicate that AL amyloid fibrils severely impair cardiomyocyte metabolism in a dose dependent manner. These data suggest that effective therapeutic intervention for these patients should include methods for removing potentially toxic amyloid fibrils.

Original languageEnglish (US)
Article numbere0137716
JournalPLoS One
Volume10
Issue number9
DOIs
StatePublished - Sep 22 2015
Externally publishedYes

Fingerprint

amyloid
Amyloid
Cardiac Myocytes
Light
amyloidosis
Confocal microscopy
Poisons
Fluorescence microscopy
cardiomyopathy
oxidoreductases
Amyloidosis
Cell death
fluorescence microscopy
Cardiomyopathies
Fluorescence Microscopy
Metabolism
Human Activities
Confocal Microscopy
Oxygen Consumption
Liver

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

McWilliams-Koeppen, H. P., Foster, J. S., Hackenbrack, N., Ramirez-Alvarado, M., Donohoe, D., Williams, A., ... Wall, J. (2015). Light chain amyloid fibrils cause metabolic dysfunction in human cardiomyocytes. PLoS One, 10(9), [e0137716]. https://doi.org/10.1371/journal.pone.0137716

Light chain amyloid fibrils cause metabolic dysfunction in human cardiomyocytes. / McWilliams-Koeppen, Helen P.; Foster, James S.; Hackenbrack, Nicole; Ramirez-Alvarado, Marina; Donohoe, Dallas; Williams, Angela; Macy, Sallie; Wooliver, Craig; Wortham, Dale; Morrell-Falvey, Jennifer; Foster, Carmen M.; Kennel, Stephen; Wall, Jonathan.

In: PLoS One, Vol. 10, No. 9, e0137716, 22.09.2015.

Research output: Contribution to journalArticle

McWilliams-Koeppen, HP, Foster, JS, Hackenbrack, N, Ramirez-Alvarado, M, Donohoe, D, Williams, A, Macy, S, Wooliver, C, Wortham, D, Morrell-Falvey, J, Foster, CM, Kennel, S & Wall, J 2015, 'Light chain amyloid fibrils cause metabolic dysfunction in human cardiomyocytes', PLoS One, vol. 10, no. 9, e0137716. https://doi.org/10.1371/journal.pone.0137716
McWilliams-Koeppen HP, Foster JS, Hackenbrack N, Ramirez-Alvarado M, Donohoe D, Williams A et al. Light chain amyloid fibrils cause metabolic dysfunction in human cardiomyocytes. PLoS One. 2015 Sep 22;10(9). e0137716. https://doi.org/10.1371/journal.pone.0137716
McWilliams-Koeppen, Helen P. ; Foster, James S. ; Hackenbrack, Nicole ; Ramirez-Alvarado, Marina ; Donohoe, Dallas ; Williams, Angela ; Macy, Sallie ; Wooliver, Craig ; Wortham, Dale ; Morrell-Falvey, Jennifer ; Foster, Carmen M. ; Kennel, Stephen ; Wall, Jonathan. / Light chain amyloid fibrils cause metabolic dysfunction in human cardiomyocytes. In: PLoS One. 2015 ; Vol. 10, No. 9.
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