Limitation of number of strains and persistence of false positive loci in QTL mapping using recombinant inbred strains

Lishi Wang, Yan Jiao, Yanhong Cao, Gaifen Liu, Yongjun Wang, Weikuan Gu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

While the identification of causal genes of quantitative trait loci (QTL) remains a difficult problem in the post-genome era, the number of QTL continues to accumulate, mainly identified using the recombinant inbred (RI) strains. Over the last decade, hundreds of publications have reported nearly a thousand QTL identified from RI strains. We hypothesized that the inaccuracy of most of these QTL makes it difficult to identify causal genes. Using data from RI strains derived from C57BL/6J (B6) X DBA/2J (D2), we tested the possibility of detection of reliable QTL with different numbers of strains in the same trait in five different traits. Our results indicated that studies using RI strains of less than 30 in general have a higher probability of failing to detect reliable QTL. Errors in many studies could include false positive loci, switches between QTL with small and major effects, and missing the real major loci. The similar data was obtained from a RI strain population derived from a different pair of parents and a RI strain population of rat. Thus, thousands of reported QTL from studies of RI strains may need to be double-checked for accuracy before proceeding to causal gene identification.

Original languageEnglish (US)
Article numbere102307
JournalPLoS ONE
Volume9
Issue number7
DOIs
StatePublished - Jul 17 2014

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Quantitative Trait Loci
quantitative trait loci
loci
Genes
Inbred Strains Rats
genes
Population
Publications
Rats
Switches
Genome
genome
rats

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Limitation of number of strains and persistence of false positive loci in QTL mapping using recombinant inbred strains. / Wang, Lishi; Jiao, Yan; Cao, Yanhong; Liu, Gaifen; Wang, Yongjun; Gu, Weikuan.

In: PLoS ONE, Vol. 9, No. 7, e102307, 17.07.2014.

Research output: Contribution to journalArticle

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AU - Gu, Weikuan

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