Limited type I interferons and plasmacytoid dendritic cells during neonatal respiratory syncytial virus infection permit immunopathogenesis upon reinfection

Stephania Cormier, Bishwas Shrestha, Jordy Saravia, Greg I. Lee, Li Shen, John Devincenzo, Young In Kim Hoehamer, Dahui You

Research output: Contribution to journalArticle

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Abstract

Respiratory syncytial virus (RSV) infection is the number one cause of bronchiolitis in infants, yet no vaccines are available because of a lack of knowledge of the infant immune system. Using a neonatal mouse model, we previously revealed that mice initially infected with RSV as neonates develop Th2-biased immunopathophysiologies during reinfection, and we demonstrated a role for enhanced interleukin-4 receptor α (IL-4Rα) expression on T helper cells in these responses. Here we show that RSV infection in neonates induced limited type I interferon (IFN) and plasmacytoid dendritic cell (pDC) responses. IFN alpha (IFN-α) treatment or adoptive transfer of adult pDCs capable of inducing IFN-α prior to neonatal RSV infection decreased Th2-biased immunopathogenesis during reinfection. A reduced viral load and downregulation of IL-4Rα on Th2 cells were observed in IFN- α-treated neonatal mice, suggesting dual mechanisms of action.

Original languageEnglish (US)
Pages (from-to)9350-9360
Number of pages11
JournalJournal of Virology
Volume88
Issue number16
DOIs
StatePublished - Jan 1 2014

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Respiratory Syncytial Virus Infections
Interferon Type I
interferons
dendritic cells
Interleukin-4 Receptors
Dendritic Cells
neonates
Interferon-alpha
viruses
Interferons
interferon-alpha
interleukin-4
Newborn Infant
infection
Th2 Cells
Bronchiolitis
Respiratory Syncytial Viruses
Adoptive Transfer
Helper-Inducer T-Lymphocytes
Viral Load

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

Limited type I interferons and plasmacytoid dendritic cells during neonatal respiratory syncytial virus infection permit immunopathogenesis upon reinfection. / Cormier, Stephania; Shrestha, Bishwas; Saravia, Jordy; Lee, Greg I.; Shen, Li; Devincenzo, John; Kim Hoehamer, Young In; You, Dahui.

In: Journal of Virology, Vol. 88, No. 16, 01.01.2014, p. 9350-9360.

Research output: Contribution to journalArticle

Cormier, Stephania ; Shrestha, Bishwas ; Saravia, Jordy ; Lee, Greg I. ; Shen, Li ; Devincenzo, John ; Kim Hoehamer, Young In ; You, Dahui. / Limited type I interferons and plasmacytoid dendritic cells during neonatal respiratory syncytial virus infection permit immunopathogenesis upon reinfection. In: Journal of Virology. 2014 ; Vol. 88, No. 16. pp. 9350-9360.
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