Linamarase expression in cassava cultivars with roots of low- and high-cyanide content

María Angélica Santana, Valeria Vasquez, Juan Matehus, Rafael Rangel Aldao

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

This paper reports the expression and localization of linamarase in roots of two cassava (Manihot esculenta Crantz) cultivars of low and high cyanide. Two different patterns of linamarase activity were observed. In the low-cyanide type, young leaves displayed very high enzyme activity during the early plant growing stage (3 months), whereas in root peel, the activity increased progressively to reach a peak in 11-month-old plants. Conversely, in the high-cyanide cultivar (HCV), root peel linamarase activity decreased during the growth cycle, whereas in expanded leaves linamarase activity peaked in 11-month-old plants. The accumulation of linamarin showed a similar pattern in both cultivars, although a higher concentration was always found in the HCV. Linamarase was found mainly in laticifer cells of petioles and roots of both cultivars with no significant differences between them. At the subcellular level, there were sharp differences because linamarase was found mainly in the cell walls of the HCV, whereas in the low-cyanide cultivar, the enzyme was present in vacuoles and cell wall of laticifer cells. Reverse transcriptase-PCR on cassava tissues showed no expression of linamarase in cassava roots, thus, the transport of linamarase from shoots to roots through laticifers is proposed.

Original languageEnglish (US)
Pages (from-to)1686-1694
Number of pages9
JournalPlant physiology
Volume129
Issue number4
DOIs
StatePublished - Aug 2 2002

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Manihot
Cyanides
cyanides
cassava
cultivars
Cell Wall
cell walls
Manihot esculenta
petioles
cyanogenic beta-glucosidase
Enzymes
Vacuoles
vacuoles
Reverse Transcriptase Polymerase Chain Reaction
leaves
reverse transcriptase polymerase chain reaction
cells
enzyme activity
shoots
enzymes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Linamarase expression in cassava cultivars with roots of low- and high-cyanide content. / Santana, María Angélica; Vasquez, Valeria; Matehus, Juan; Aldao, Rafael Rangel.

In: Plant physiology, Vol. 129, No. 4, 02.08.2002, p. 1686-1694.

Research output: Contribution to journalArticle

Santana, María Angélica ; Vasquez, Valeria ; Matehus, Juan ; Aldao, Rafael Rangel. / Linamarase expression in cassava cultivars with roots of low- and high-cyanide content. In: Plant physiology. 2002 ; Vol. 129, No. 4. pp. 1686-1694.
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