Lipoprotein and apolipoprotein secretion by a newborn piglet intestinal cell line (IPEC-1)

Ruben Gonzalez-Vallina, Heng Wang, Reggie Zhan, Helen M. Berschneider, Robert M. Lee, Nicholas O. Davidson, Dennis Black

Research output: Contribution to journalArticle

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Abstract

The aim of these studies was to characterize the synthesis and secretion of lipoproteins and apolipoprotein B (apo B) and apo A-I by a newborn swine intestinal epithelial cell line (IPEC-1). Differentiated cells exhibited enterocytic features, including microvilli. [:3Hloleic acid was taken up and incorporated into cellular lipids and secreted into the basolateral medium in lipoproteins. Total apo B and apo A-I secreted increased with oleic acid incubation. However, cellular apo B and apo A-I content did not change. Whereas undifferentiated cells synthesized and secreted only apo B-100, both apo B-100 and apo B-48 were produced by differentiated cells. The ratio of radiolabeled apo B-48 to ape B-100 in both basolateral medium and cell homogenate increased with oleic acid treatment after 24-h steady-state labeling. However, apo B mRNA editing was unchanged, indicating posttranslational regulation of this ratio. Pulse-chase radiolabeling demonstrated no major changes in cellular or basolateral medium apolipoprotein labeling kinetics with oleic acid or dexamethasone incubation. The dissociation of apo B and apo A-I mass secretion from the secretion of radiolabeled apo B and apo A-I in response to oleic acid absorption suggests the presence of an intracellular pool of apolipoprotein with a slow turnover that is mobilized for secretion in response to fatty acid uptake.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume271
Issue number2 34-2
StatePublished - Aug 1 1996

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Apolipoproteins
Apolipoproteins B
Apolipoprotein A-I
Lipoproteins
Oleic Acid
Cell Line
Apolipoprotein B-48
Apolipoprotein B-100
Hominidae
Microvilli
Dexamethasone
Swine
Fatty Acids
Epithelial Cells
Lipids
Messenger RNA
Acids

All Science Journal Classification (ASJC) codes

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

Gonzalez-Vallina, R., Wang, H., Zhan, R., Berschneider, H. M., Lee, R. M., Davidson, N. O., & Black, D. (1996). Lipoprotein and apolipoprotein secretion by a newborn piglet intestinal cell line (IPEC-1). American Journal of Physiology - Gastrointestinal and Liver Physiology, 271(2 34-2).

Lipoprotein and apolipoprotein secretion by a newborn piglet intestinal cell line (IPEC-1). / Gonzalez-Vallina, Ruben; Wang, Heng; Zhan, Reggie; Berschneider, Helen M.; Lee, Robert M.; Davidson, Nicholas O.; Black, Dennis.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 271, No. 2 34-2, 01.08.1996.

Research output: Contribution to journalArticle

Gonzalez-Vallina R, Wang H, Zhan R, Berschneider HM, Lee RM, Davidson NO et al. Lipoprotein and apolipoprotein secretion by a newborn piglet intestinal cell line (IPEC-1). American Journal of Physiology - Gastrointestinal and Liver Physiology. 1996 Aug 1;271(2 34-2).
Gonzalez-Vallina, Ruben ; Wang, Heng ; Zhan, Reggie ; Berschneider, Helen M. ; Lee, Robert M. ; Davidson, Nicholas O. ; Black, Dennis. / Lipoprotein and apolipoprotein secretion by a newborn piglet intestinal cell line (IPEC-1). In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1996 ; Vol. 271, No. 2 34-2.
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