Liposome-encapsulated curcumin suppresses neuroblastoma growth through nuclear factor-kappa B inhibition

Wayne S. Orr, Jason W. Denbo, Karim R. Saab, Adrianne L. Myers, Catherine Y. Ng, Junfang Zhou, Christopher L. Morton, Lawrence Pfeffer, Andrew M. Davidoff

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: Nuclear factor-κB (NF-κB) has been implicated in tumor cell proliferation and survival and in tumor angiogenesis. We sought to evaluate the effects of curcumin, an inhibitor of NF-κB, on a xenograft model of disseminated neuroblastoma. Methods: For in vitro studies, neuroblastoma cell lines NB1691, CHLA-20, and SK-N-AS were treated with various doses of liposomal curcumin. Disseminated neuroblastoma was established in vivo by tail vein injection of NB1691-luc cells into SCID mice, which were then treated with 50 mg/kg/day of liposomal curcumin 5 days/week intraperitoneally. Results: Curcumin suppressed NF-κB activation and proliferation of all neuroblastoma cell lines in vitro. In vivo, curcumin treatment resulted in a significant decrease in disseminated tumor burden. Curcumin-treated tumors had decreased NF-κB activity and an associated significant decrease in tumor cell proliferation and an increase in tumor cell apoptosis, as well as a decrease in tumor vascular endothelial growth factor levels and microvessel density. Conclusion: Liposomal curcumin suppressed neuroblastoma growth, with treated tumors showing a decrease in NF-κB activity. Our results suggest that liposomal curcumin may be a viable option for the treatment of neuroblastoma that works via inhibiting the NF-κB pathway.

Original languageEnglish (US)
Pages (from-to)736-744
Number of pages9
JournalSurgery
Volume151
Issue number5
DOIs
StatePublished - May 1 2012

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Curcumin
NF-kappa B
Neuroblastoma
Liposomes
Growth
Neoplasms
Cell Proliferation
Cell Line
SCID Mice
Microvessels
Tumor Burden
Heterografts
Vascular Endothelial Growth Factor A
Tail
Veins
Cell Survival
Apoptosis
Injections
Therapeutics

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Orr, W. S., Denbo, J. W., Saab, K. R., Myers, A. L., Ng, C. Y., Zhou, J., ... Davidoff, A. M. (2012). Liposome-encapsulated curcumin suppresses neuroblastoma growth through nuclear factor-kappa B inhibition. Surgery, 151(5), 736-744. https://doi.org/10.1016/j.surg.2011.12.014

Liposome-encapsulated curcumin suppresses neuroblastoma growth through nuclear factor-kappa B inhibition. / Orr, Wayne S.; Denbo, Jason W.; Saab, Karim R.; Myers, Adrianne L.; Ng, Catherine Y.; Zhou, Junfang; Morton, Christopher L.; Pfeffer, Lawrence; Davidoff, Andrew M.

In: Surgery, Vol. 151, No. 5, 01.05.2012, p. 736-744.

Research output: Contribution to journalArticle

Orr, WS, Denbo, JW, Saab, KR, Myers, AL, Ng, CY, Zhou, J, Morton, CL, Pfeffer, L & Davidoff, AM 2012, 'Liposome-encapsulated curcumin suppresses neuroblastoma growth through nuclear factor-kappa B inhibition', Surgery, vol. 151, no. 5, pp. 736-744. https://doi.org/10.1016/j.surg.2011.12.014
Orr, Wayne S. ; Denbo, Jason W. ; Saab, Karim R. ; Myers, Adrianne L. ; Ng, Catherine Y. ; Zhou, Junfang ; Morton, Christopher L. ; Pfeffer, Lawrence ; Davidoff, Andrew M. / Liposome-encapsulated curcumin suppresses neuroblastoma growth through nuclear factor-kappa B inhibition. In: Surgery. 2012 ; Vol. 151, No. 5. pp. 736-744.
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