Liposome-mediated transfection.

Michael Whitt, L. Buonocore, J. K. Rose

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Using liposomes to deliver DNA into different eukaryotic cell types results in higher efficiency and greater reproducibility than other transfection methods. In this unit, Basic Protocol the describes a transient expression system while an Alternate Protocol involves stable transformation and expression of DNA integrated into the genome of the transfected cell. In both protocols, plasmid DNA derived from either crude (miniprep) or purified (through CsCl) preparations is mixed with a liposome suspension comprised of cationic lipids and applied to monolayer cell cultures.

Original languageEnglish (US)
JournalCurrent protocols in immunology / edited by John E. Coligan ... [et al.]
VolumeChapter 10
StatePublished - Jan 1 2001

Fingerprint

Liposomes
Transfection
DNA
Eukaryotic Cells
Suspensions
Plasmids
Cell Culture Techniques
Genome
Lipids
cesium chloride

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Liposome-mediated transfection. / Whitt, Michael; Buonocore, L.; Rose, J. K.

In: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 10, 01.01.2001.

Research output: Contribution to journalArticle

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