Live, attenuated influenza virus (LAIV) vehicles are strong inducers of immunity toward influenza B virus

Victor C. Huber, Loren H. Kleimeyer, Jonathan Mccullers

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Historically, vaccines developed toward influenza viruses of the B type using methodologies developed for influenza A viruses as a blueprint have not been equally efficacious or effective. Because most influenza research and public attention concerns influenza A viruses, these shortcomings have not been adequately addressed. In this manuscript, we utilized different influenza vaccine vehicles to compare immunogenicity and protection in mice and ferrets after vaccination against an influenza B virus. We report that plasmid DNA vaccines demonstrate low immunogenicity profiles and poor protection compared to either whole, inactivated influenza virus (IIV) or, live, attenuated influenza virus (LAIV) vaccines. When mixed prime:boost regimens using LAIV and IIV were studied, we observed a boosting effect in mice after priming with LAIV that was not seen when IIV was used as the prime. In ferrets LAIV induced high antibody titers after a single dose and provided a boost in IIV-primed animals. Regimens including LAIV as a prime demonstrated enhanced protection, and adjuvantation was required for efficacy using the IIV preparation. Our results differ from generally accepted influenza A virus vaccine models, and argue that strategies for control of influenza B virus should be considered separately from those for influenza A virus.

Original languageEnglish (US)
Pages (from-to)5381-5388
Number of pages8
JournalVaccine
Volume26
Issue number42
DOIs
StatePublished - Oct 3 2008

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Influenza B virus
Orthomyxoviridae
Immunity
immunity
Influenza A virus
Influenza Vaccines
vaccines
Ferrets
ferrets
influenza
immune response
Attenuated Vaccines
DNA Vaccines
mice
recombinant vaccines
Human Influenza
Vaccination
Plasmids
Vaccines
plasmids

All Science Journal Classification (ASJC) codes

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Live, attenuated influenza virus (LAIV) vehicles are strong inducers of immunity toward influenza B virus. / Huber, Victor C.; Kleimeyer, Loren H.; Mccullers, Jonathan.

In: Vaccine, Vol. 26, No. 42, 03.10.2008, p. 5381-5388.

Research output: Contribution to journalArticle

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